ArticlePlant physiology2020
Rapid Affinity Purification of Tagged Plant Mitochondria (Mito-AP) for Metabolome and Proteome Analyses.
Article in Plant physiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 59 citations in OpenAlex.
- Illuminating the subcellular maze: fluorescence-activated organelle sorting in plant sciences.Journal of experimental botany · 2025Review
- The complex I subunit B22 contains a LYR domain that is crucial for an interaction with the mitochondrial acyl carrier protein SDAP1.The Plant journal : for cell and molecular biology · 2025Article
- Synthesis of β-Alanine From Isoleucine and Propionate Catabolism via Aminotransferases.Plant direct · 2024Article
- Three Arabidopsis UMP kinases have different roles in pyrimidine nucleotide biosynthesis and (deoxy)CMP salvage.The Plant cell · 2024Article
- The evolution of strictly monofunctional naphthoquinol C-methyltransferases is vital in cyanobacteria and plastids.The Plant cell · 2023Article
- Characterization ofMetabolites · 2023Article
- Defining the lipidome of Arabidopsis leaf mitochondria: Specific lipid complement and biosynthesis capacity.Plant physiology · 2023Article
- Mitochondrial ferredoxin-like is essential for forming complex I-containing supercomplexes in Arabidopsis.Plant physiology · 2023Article
- The nucleotide metabolome of germinating Arabidopsis thaliana seeds reveals a central role for thymidine phosphorylation in chloroplast development.The Plant cell · 2022Article
- Isolation of Plant Mitochondria Using Affinity Purification.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Rapid Affinity Purification of Tagged Plant Mitochondria (Mito-AP).Methods in molecular biology (Clifton, N.J.) · 2022Article
- Construction of Mitochondrial Protection and Monitoring Model of Lon Protease Based on Machine Learning under Myocardial Ischemia Environment.Journal of environmental and public health · 2022Article
- Review
- Article
- Auxin Metabolome Profiling in the Arabidopsis Endoplasmic Reticulum Using an Optimised Organelle Isolation Protocol.International journal of molecular sciences · 2021Article
- Enhanced nucleotide analysis enables the quantification of deoxynucleotides in plants and algae revealing connections between nucleoside and deoxynucleoside metabolism.The Plant cell · 2021Article
- Analysis of Nucleosides and Nucleotides in Plants: An Update on Sample Preparation and LC-MS Techniques.Cells · 2021Review
- Isolation and comparative proteomic analysis of mitochondria from the pulp of ripening citrus fruit.Horticulture research · 2021Article
- Plant expression of NifD protein variants resistant to mitochondrial degradation.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Matrix Redox Physiology Governs the Regulation of Plant Mitochondrial Metabolism through Posttranslational Protein Modifications.The Plant cell · 2020Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The isolation of organelles facilitates the focused analysis of subcellular protein and metabolite pools. Here we present a technique for the affinity purification of plant mitochondria (Mito-AP). The stable ectopic expression of a mitochondrial outer membrane protein fused to a GFP:Strep tag in Arabidopsis (
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