ArticleProceedings of the National Academy of Sciences of the United States of America2020
Intermediate states of molecular self-assembly from liquid-cell electron microscopy.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 68 citations in OpenAlex.
- Kinetics and dynamics of oligonucleotide hybridization.Nature reviews. Chemistry · 2025Review
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- Understanding, Mimicking, and Mitigating Radiolytic Damage to Polymers in Liquid Phase Transmission Electron Microscopy.Advanced materials (Deerfield Beach, Fla.) · 2024Article
- Nanopipette dynamic microscopy unveils nano coffee ring.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Electron videography of a lipid-protein tango.Science advances · 2024Article
- The ergodicity question when imaging DNA conformation using liquid cell electron microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Visualizing the multi-level assembly structures of conjugated molecular systems with chain-length dependent behavior.Nature communications · 2023Article
- Real-time imaging of sulfhydryl single-stranded DNA aggregation.Communications chemistry · 2023Article
- Liquid-cell transmission electron microscopy for imaging of thermosensitive recombinant polymers.Journal of controlled release : official journal of the Controlled Release Society · 2022Article
- Coordination Cage-Based Emulsifiers: Templated Formation of Metal Oxide Microcapsules Monitored by In Situ LC-TEM.Chemistry (Weinheim an der Bergstrasse, Germany) · 2022Article
- Multi-step atomic mechanism of platinum nanocrystals nucleation and growth revealed by in-situ liquid cell STEM.Scientific reports · 2021Article
- Emergence and Rearrangement of Dynamic Supramolecular Aggregates Visualized by Interferometric Scattering Microscopy.ACS nano · 2020Article
- Ultrafast electron imaging of surface charge carrier dynamics at low voltage.Structural dynamics (Melville, N.Y.) · 2020Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Traditional single-molecule methods do not report whole-molecule kinetic conformations, and their adaptive shape changes during the process of self-assembly. Here, using graphene liquid-cell electron microscopy with electrons of low energy at low dose, we show that this approach resolves the time dependence of conformational adaptations of macromolecules for times up to minutes, the resolution determined by motion blurring, with DNA as the test case. Single-stranded DNA molecules are observed in real time as they hybridize near the solid surface to form double-stranded helices; we contrast molecules the same length but differing in base-pair microstructure (random, blocky, and palindromic hairpin) whose key difference is that random sequences possess only one stable final state, but the others offer metastable intermediate structures. Hybridization is observed to couple with enhanced translational mobility and torsion-induced rotation of the molecule. Prevalent transient loops are observed in error-correction processes. Transient melting and other failed encounters are observed in the competitive binding of multiple single-stranded molecules. Among the intermediate states reported here, some were predicted but not observed previously, and the high incidence of looping and enhanced mobility come as surprises. The error-producing mechanisms, failed encounters, and transient intermediate states would not be easily resolved by traditional single-molecule methods. The methods generalize to visualize motions and interactions of other organic macromolecules.
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