Evidence map›Paper›PMID 31911225›Full record

ArticleJournal of colloid and interface science2020

Engineering magnetic nanoparticles and their integration with microfluidics for cell isolation.

Mythreyi Unni, Jinling Zhang, Thomas J George, Mark S Segal, Z Hugh Fan, Carlos Rinaldi

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of colloid and interface science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed, 1 pooled it
1.6field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 1 synthesis or guideline pooled it, 40 citations in OpenAlex.

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  7. FeMaterials (Basel, Switzerland) · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Mythreyi UnniDepartment of Chemical Engineering, Gainesville, FL 32611, USA.
Jinling ZhangInterdisciplinary Microsystems Group, Department of Mechanical and Aerospace Engineering, Gainesville, FL 32611, USA.
Thomas J GeorgeDepartment of Medicine, Gainesville, FL 32611, USA; University of Florida Health Cancer Center, Gainesville, FL 32611, USA.
Mark S SegalDepartment of Medicine, Gainesville, FL 32611, USA.
Z Hugh FanInterdisciplinary Microsystems Group, Department of Mechanical and Aerospace Engineering, Gainesville, FL 32611, USA; J. Crayton Pruitt Family Department of Biomedical Engineering, Gainesville, FL 32611, USA; Department of Chemistry, University of Florida, Gainesville, FL 32611, USA; University of Florida Health Cancer Center, Gainesville, FL 32611, USA. Electronic address: hfan@ufl.edu.
Carlos RinaldiDepartment of Chemical Engineering, Gainesville, FL 32611, USA; J. Crayton Pruitt Family Department of Biomedical Engineering, Gainesville, FL 32611, USA; University of Florida Health Cancer Center, Gainesville, FL 32611, USA. Electronic address: carlos.rinaldi@ufl.edu.
University of Florida Health · USUniversity of Florida · USInterdisciplinary Consulting Corporation (United States) · US

Funding

Mentored Quantitative Research Career Development AwardK25CA149080 · NCI · UNIVERSITY OF FLORIDA · PI FAN, Z. HUGH · 2011 to 2015
$897k
NCI NIH HHS K25 CA149080
6 · The paper itself

Abstract

Isolation of cancer cells, bacteria, and viruses from peripheral blood has important applications in cancer diagnosis, therapy monitoring, and drug development. Magnetic particles functionalized with antibodies that target receptors of cancer cells have been shown to isolate such entities using magnetic field gradients. Here, we report enhancement in capture efficiency and specificity by engineering magnetic nanoparticles and integrating them with microfluidics for the enumeration of tumor cells. Nanoparticles were made from iron oxide, coated with poly(ethylene glycol), and conjugated through avidin-biotin chemistry with antibody specifically against epithelial cell adhesion molecule (EpCAM). On exposure of targeted nanoparticles to tumor cells, specific uptake by EpCAM-expressing tumor cells (e.g., BxPC3, a pancreatic cancer cell) was observed, whereas there was negligible uptake by cells with low EpCAM expression (e.g., CCRF-CEM, a leukemia cell). Using an arrangement of magnets called a Halbach array, capture efficiency and specificity towards BxPC3 cells tagged with magnetic nanoparticles were enhanced, compared to conditions without the magnetic field gradient and/or without magnetic nanoparticles, either in buffer or in whole blood. These results illustrate that engineered magnetic nanoparticles and their integration with microfluidics have great potential for tumor cell enumeration and cancer prognosis.

Indexed as

Cell SeparationLab-On-A-Chip DevicesMicrofluidic Analytical TechniquesCell Line, TumorEpithelial Cell Adhesion MoleculeHumansMagnetite NanoparticlesNeoplasm ProteinsNeoplastic Cells, CirculatingEpithelial Cell Adhesion MoleculeMagnetite NanoparticlesNeoplasm ProteinsCaptureMagnetophoresisMicrofluidic deviceTargeted streptavidin magnetic nanoparticlesTumor cells

Identifiers

PMID31911225
PMCPMC7023483
OpenAlexW2997515035

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.