Evidence map›Paper›PMID 31910830›Full record

ArticleMalaria journal2020

Identification of Plasmodium falciparum proteoforms from liver stage models.

Benjamin Winer, Kimberly A Edgel, Xiaoyan Zou, Julie Sellau, Sri Hadiwidjojo, Lindsey S Garver, Christin E McDonough, Neil L Kelleher, Paul M Thomas, Eileen Villasante and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Malaria journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 5 institutions in 2 countries.

Benjamin WinerDepartment of Molecular Biology, Princeton University, Washington Road, Princeton, NJ, 08544, USA.
Kimberly A EdgelNaval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD, 20910, USA.
Xiaoyan ZouNaval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD, 20910, USA.
Julie SellauDepartment of Molecular Biology, Princeton University, Washington Road, Princeton, NJ, 08544, USA.
Sri HadiwidjojoNaval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD, 20910, USA.
Lindsey S GarverWalter Reed Army Institute of Research, 503 Robert Grant Avenue, Silver Spring, MD, 20190, USA.
Christin E McDonoughThe Henry M Jackson Foundation, 6720A Rockledge Dr., Rockville, MD, 20817, USA.
Neil L KelleherNorthwestern University National Resource for Translational Proteomics, Evanston, IL, 60208, USA.
Paul M ThomasNorthwestern University National Resource for Translational Proteomics, Evanston, IL, 60208, USA.
Eileen VillasanteNaval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD, 20910, USA.
Alexander PlossDepartment of Molecular Biology, Princeton University, Washington Road, Princeton, NJ, 08544, USA. aploss@princeton.edu.
Vincent R GerbasiNaval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD, 20910, USA. robert.gerbasi@northwestern.edu.
Naval Medical Research Command · USNorthwestern University · USPrinceton University · USJackson Foundation · USWalter Reed Army Institute of Research · US

Funding

PREDOCTORAL TRAINING PROGRAM IN GENETICST32GM007388 · NIGMS · PRINCETON UNIVERSITY · PI CRISTEA, ILEANA M. · 1985 to 2022
$27.4M
TR&D 7: Cell Specific ProteomicsP41GM108569 · NIGMS · NORTHWESTERN UNIVERSITY · PI KELLEHER, NEIL L · 2015 to 2024
$13.6M
Bill and Melinda Gates Foundation OP1119033Burroughs Wellcome Fund 1015389NIGMS NIH HHS GM108569NIGMS NIH HHS P41 GM108569NIGMS NIH HHS T32 GM007388
6 · The paper itself

Abstract

backgroundImmunization with attenuated malaria sporozoites protects humans from experimental malaria challenge by mosquito bite. Protection in humans is strongly correlated with the production of T cells targeting a heterogeneous population of pre-erythrocyte antigen proteoforms, including liver stage antigens. Currently, few T cell epitopes derived from Plasmodium falciparum, the major aetiologic agent of malaria in humans are known.

methodsIn this study both in vitro and in vivo malaria liver stage models were used to sequence host and pathogen proteoforms. Proteoforms from these diverse models were subjected to mild acid elution (of soluble forms), multi-dimensional fractionation, tandem mass spectrometry, and top-down bioinformatics analysis to identify proteoforms in their intact state.

resultsThese results identify a group of host and malaria liver stage proteoforms that meet a 5% false discovery rate threshold.

conclusionsThis work provides proof-of-concept for the validity of this mass spectrometry/bioinformatic approach for future studies seeking to reveal malaria liver stage antigens towards vaccine development.

Indexed as

AnimalsAntigens, ProtozoanDisease Models, AnimalEpitopes, T-LymphocyteFemaleHepatocytesImmunity, CellularLiverMalaria, FalciparumMalaria VaccinesMass SpectrometryMicePlasmodium falciparumProteomicsSerum Albumin, HumanAntigens, ProtozoanEpitopes, T-LymphocyteMalaria VaccinesSerum Albumin, HumanAntigenCell-mediated immunityLiver stageProteomicsTop-downVaccine

Identifiers

PMID31910830
PMCPMC6947969
OpenAlexW3002131828

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.