ArticleSensors and actuators. B, Chemical1997
Optical assay for glucose based on the luminescnence decay time of the long wavelength dye Cy5™.
Article in Sensors and actuators. B, Chemical, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 95 citations in OpenAlex.
- Article
- Noninvasive glucose detection in exhaled breath condensate.Translational research : the journal of laboratory and clinical medicine · 2019Review
- A Layer-by-Layer Approach To Retain a Fluorescent Glucose Sensing Assay within the Cavity of a Hydrogel Membrane.ACS applied bio materials · 2018Article
- Overcoming the aggregation problem: a new type of fluorescent ligand for ConA-based glucose sensing.Biosensors & bioelectronics · 2015Article
- PEGylation of concanavalin A to improve its stability for an in vivo glucose sensing assay.Analytical chemistry · 2014Article
- Acute in vivo performance evaluation of the fluorescence affinity sensor in the intravascular and interstitial space in Swine.Journal of diabetes science and technology · 2013Article
- A human pilot study of the fluorescence affinity sensor for continuous glucose monitoring in diabetes.Journal of diabetes science and technology · 2012Article
- Encapsulation of a Concanavalin A/dendrimer glucose sensing assay within microporated poly (ethylene glycol) microspheres.Biomedical optics express · 2011Article
- Glucose sensing by time-resolved fluorescence of sol-gel immobilized glucose oxidase.Sensors (Basel, Switzerland) · 2011Article
- Preclinical in vivo study of a fluorescence affinity sensor for short-term continuous glucose monitoring in a small and large animal model.Diabetes technology & therapeutics · 2008Article
- Fiber-coupled fluorescence affinity sensor for 3-day in vivo glucose sensing.Journal of diabetes science and technology · 2007Article
- Two-photon excited fluorescence energy transfer: a study based on oligonucleotide rulers.Journal of fluorescence · 2006Article
- Article
- A glucose-sensing contact lens: from bench top to patient.Current opinion in biotechnology · 2005Review
- Boronic acid fluorescent sensors for monosaccharide signaling based on the 6-methoxyquinolinium heterocyclic nucleus: progress toward noninvasive and continuous glucose monitoring.Bioorganic & medicinal chemistry · 2005Article
- OPHTHALMIC GLUCOSE MONITORING USING DISPOSABLE CONTACT LENSES.Reviews in fluorescence · 2005Article
- Protein-based biosensors for diabetic patients.Journal of fluorescence · 2004Article
- Glucose-sensitive nanoassemblies comprising affinity-binding complexes trapped in fuzzy microshells.Journal of fluorescence · 2004Article
- Ophthalmic glucose monitoring using disposable contact lenses--a review.Journal of fluorescence · 2004Review
- Fluorescence glucose detection: advances toward the ideal in vivo biosensor.Journal of fluorescence · 2004Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
An optical assay for glucose is described based on the luminescence decay time of a long wavelength dye (Cy5) which can be excited with currently available red laser diodes. Concanavalin A was covalently labeled with Cy5 which served as the donor in an assay based on fluorescence resonance energy transfer (FRET). The acceptor was Malachite Green which was covalently linked to insulin which served as a carrier protein. To provide binding affinity for ConA Malachite Green insulin was also covalently labeled with maltose (MIMG). Binding of Cy5ConA to MIMG resulted in a decreased intensity and decay time of Cy5 as observed by time-correlated single photon counting. Glucose was detected by competitive displacement of MIMG from Cy5ConA, resulting in increased intensity and decay time. This glucose assay has several features which can result in practical real world assays for glucose. The long absorption wavelength of Cy5 allows excitation with red laser diodes, which can be readily pulsed or amplitude-modulated for time-domain or frequency-domain decay time measurements. Additionally, decay times can be measured through skin using long wavelength excitation and emission, suggesting the possibility of an implanted glucose sensor. And finally, the assay affinity and reversibility can in principle be adjusted by controlling the extent and type of sugar labeling of the carrier protein.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.