ArticleMolecular plant pathology2020
Discriminant haplotypes of avirulence genes of Phytophthora sojae lead to a molecular assay to predict phenotypes.
Article in Molecular plant pathology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 20 citations in OpenAlex.
- A global-temporal analysis on Phytophthora sojae resistance-gene efficacy.Nature communications · 2023Pooled it
- Targeted capture and cloning of Rps6 reveal redundancy in soybean resistance genes.BMC plant biology · 2026Article
- Genetic diversity and evolution of nine avirulence genes in South KoreanFrontiers in microbiology · 2026Article
- Allelism of Rps3b and Rps11 revealed by NLR gene capture of resistance genes to Phytophthora sojae in soybean.The plant genome · 2025Article
- Pathotypes and Simple Sequence Repeat (SSR)-Based Genetic Diversity ofMicroorganisms · 2025Article
- Development and application of aFrontiers in plant science · 2025Article
- Progress and Challenges in Elucidating the Functional Role of Effectors in the Soybean-Journal of fungi (Basel, Switzerland) · 2022Review
- RXLR effector gene Avr3a from Phytophthora sojae is recognized by Rps8 in soybean.Molecular plant pathology · 2022Article
- Mapping of partial resistance to Phytophthora sojae in soybean PIs using whole-genome sequencing reveals a major QTL.The plant genome · 2022Article
- The SoyaGen Project: Putting Genomics to Work for Soybean Breeders.Frontiers in plant science · 2022Review
- Mutations in the Promoter and Coding Regions ofFrontiers in microbiology · 2021Article
- The Histological, Effectoromic, and Transcriptomic Analyses ofInternational journal of molecular sciences · 2020Article
- Discriminant haplotypes of avirulence genes of Phytophthora sojae lead to a molecular assay to predict phenotypes.Molecular plant pathology · 2020Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
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No grant is acknowledged in the PubMed record.
Abstract
The soybean-Phytophthora sojae interaction operates on a gene-for-gene relationship, where the product of a resistance gene (Rps) in the host recognizes that of an avirulence gene (Avr) in the pathogen to generate an incompatible reaction. To exploit this form of resistance, one must match with precision the appropriate Rps gene with the corresponding Avr gene. Currently, this association is evaluated by phenotyping assays that are labour-intensive and often imprecise. To circumvent this limitation, we sought to develop a molecular assay that would reveal the avirulence allele of the seven main Avr genes (Avr1a, Avr1b, Avr1c, Avr1d, Avr1k, Avr3a, and Avr6) in order to diagnose with precision the pathotypes of P. sojae isolates. For this purpose, we analysed the genomic regions of these Avr genes in 31 recently sequenced isolates with different virulence profiles and identified discriminant mutations between avirulence and virulence alleles. Specific primers were designed to generate amplicons of a distinct size, and polymerase chain reaction conditions were optimized in a final assay of two parallel runs. When tested on the 31 isolates of known virulence, the assay accurately revealed all avirulence alleles. The test was further assessed and compared to a phenotyping assay on 25 isolates of unknown virulence. The two assays matched in 97% (170/175) of the interactions studied. Interestingly, the sole cases of discrepancy were obtained with Avr3a, which suggests a possible imperfect interaction with Rps3a. This molecular assay offers a powerful and reliable tool to exploit and study with greater precision soybean resistance against P. sojae.
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