ArticleNature methods2020
Nanoscale subcellular architecture revealed by multicolor three-dimensional salvaged fluorescence imaging.
Article in Nature methods, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers, 1 of them a synthesis that pooled it.
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Who cites it
60 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Stay in touch with the endoplasmic reticulum.Science China. Life sciences · 2024Pooled it
- Aberration-aware 3D localization microscopy via self-supervised neural-physics learning.Nature communications · 2026Article
- Electrochemical Dye Switching Assisted Spectral Demixing and 3D STORM Imaging.Angewandte Chemie (International ed. in English) · 2026Article
- Mirror-enhanced 4Pi-SMLM with one objective enables isotropic nanoscale imaging.Nature biotechnology · 2026Article
- Fluorogenic speed-optimized DNA-PAINT probes enable super-resolution imaging of whole cells.bioRxiv : the preprint server for biology · 2026Article
- Automated Cyclic Super-Resolution Microscopy for Nanoscale Protein Mapping.bioRxiv : the preprint server for biology · 2026Article
- DNA-PAINT resolves E-cadherin-independent cross-junctional F-actin organization in Drosophila embryonic tissue.Biophysical journal · 2026Article
- Molecular-scale isotropic 3D super-resolution microscopy via interference localization.Nature methods · 2026Article
- 4Pi-SIMFLUX: 4Pi single-molecule localization microscopy with structured illumination.Nature methods · 2026Article
- Simulating Multicolor Super-Resolution Imaging Using an RGB Camera.Computational and structural biotechnology journal · 2026Article
- ARMH2 is a cytosolic component of CatSper crucial for sperm function.Nature communications · 2025Article
- Cellular optical imaging techniques: a dynamic advancing frontier.Science China. Life sciences · 2025Review
- Advancing Single-Molecule Biophysics: Next-Generation Organic Fluorophores with Tailored Labeling Strategies.Chemical & biomedical imaging · 2025Review
- TUG protein acts through a disordered region to organize the early secretory pathway.Nature communications · 2025Article
- Isotropic 100-nm resolution live-cell imaging with 4Pi-SIM.Nature methods · 2025Article
- Elucidating subcellular architecture and dynamics at isotropic 100-nm resolution with 4Pi-SIM.Nature methods · 2025Article
- Phasor-FSTM: a new paradigm for multicolor super-resolution imaging of living cells based on fluorescence modulation and lifetime multiplexing.Light, science & applications · 2025Article
- Fortunate molecules boost signal to background ratio and localization precision in correlation based single molecule localization microscopy.Communications biology · 2024Article
- VPS13B is localized at the interface between Golgi cisternae and is a functional partner of FAM177A1.The Journal of cell biology · 2024Article
- Multiplexed Nanoscopy via Buffer Exchange.ACS nano · 2024Article
Corrections and comments
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Authors and funding
15 authors.
Funding
Abstract
Combining the molecular specificity of fluorescent probes with three-dimensional imaging at nanoscale resolution is critical for investigating the spatial organization and interactions of cellular organelles and protein complexes. We present a 4Pi single-molecule switching super-resolution microscope that enables ratiometric multicolor imaging of mammalian cells at 5-10-nm localization precision in three dimensions using 'salvaged fluorescence'. Imaging two or three fluorophores simultaneously, we show fluorescence images that resolve the highly convoluted Golgi apparatus and the close contacts between the endoplasmic reticulum and the plasma membrane, structures that have traditionally been the imaging realm of electron microscopy. The salvaged fluorescence approach is equally applicable in most single-objective microscopes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.