ArticleNature methods2020
Template-free detection and classification of membrane-bound complexes in cryo-electron tomograms.
Article in Nature methods, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 92 citations in OpenAlex.
- Graph identification of proteins in tomograms (GRIP-Tomo) 2.0: Topologically aware classification for proteins.Protein science : a publication of the Protein Society · 2026Article
- Semi-supervised clustering with knowledge-guided representation learning in cryo-electron tomography.PLOS digital health · 2026Article
- Surface Morphometrics reveals local membrane thickness variation in organellar subcompartments.The Journal of cell biology · 2026Article
- Prion-like MAVS fibrils stitch mitochondria to promote a rapid antiviral response.Cellular & molecular immunology · 2026Article
- Article
- PickET: An unsupervised method for localizing macromolecules in cryo-electron tomograms.Research square · 2025Article
- Template Learning: Deep learning with domain randomization for particle picking in cryo-electron tomography.Nature communications · 2025Article
- PickET: An unsupervised method for localizing macromolecules in cryo-electron tomograms.bioRxiv : the preprint server for biology · 2025Article
- A practical look at cryo-electron tomography image processing: Key considerations for new biological discoveries.Current opinion in structural biology · 2025Review
- A noise-robust classification method for cryo-ET subtomograms with out-of-distribution detection.Bioinformatics (Oxford, England) · 2025Article
- Surface morphometrics reveals local membrane thickness variation in organellar subcompartments.bioRxiv : the preprint server for biology · 2025Article
- In situ and in vitro cryo-EM reveal structures of mycobacterial encapsulin assembly intermediates.Communications biology · 2025Article
- Cryo-ET suggests tubulin chaperones form a subset of microtubule lumenal particles with a role in maintaining neuronal microtubules.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- MPicker: visualizing and picking membrane proteins for cryo-electron tomography.Nature communications · 2025Article
- Article
- Native architecture of a human GBP1 defense complex for cell-autonomous immunity to infection.Science (New York, N.Y.) · 2024Article
- Article
- Molecular view of ER membrane remodeling by the Sec61/TRAP translocon.EMBO reports · 2023Article
- Atg18 oligomer organization in assembled tubes and on lipid membrane scaffolds.Nature communications · 2023Article
- Charting the molecular landscape of the cell.Structure (London, England : 1993) · 2023Review
Corrections and comments
- Erratum issued
- Erratum issued
Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
With faithful sample preservation and direct imaging of fully hydrated biological material, cryo-electron tomography provides an accurate representation of molecular architecture of cells. However, detection and precise localization of macromolecular complexes within cellular environments is aggravated by the presence of many molecular species and molecular crowding. We developed a template-free image processing procedure for accurate tracing of complex networks of densities in cryo-electron tomograms, a comprehensive and automated detection of heterogeneous membrane-bound complexes and an unsupervised classification (PySeg). Applications to intact cells and isolated endoplasmic reticulum (ER) allowed us to detect and classify small protein complexes. This classification provided sufficiently homogeneous particle sets and initial references to allow subsequent de novo subtomogram averaging. Spatial distribution analysis showed that ER complexes have different localization patterns forming nanodomains. Therefore, this procedure allows a comprehensive detection and structural analysis of complexes in situ.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.