ArticleBMC infectious diseases2020
ELISA is superior to bacterial culture and agglutination test in the diagnosis of brucellosis in an endemic area in China.
Article in BMC infectious diseases, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.
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Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it, 48 citations in OpenAlex.
- Diagnosis of human brucellosis: Systematic review and meta-analysis.PLoS neglected tropical diseases · 2024Pooled it
- Brucellosis in the One Health Context: Gaps in Diagnosis, Control, and Global Epidemiology.Journal of epidemiology and global health · 2026Review
- Cervical brucellar spondylitis: Current management strategies and future directions.Infectious medicine · 2026Review
- Evaluating a pathogen-specific IgG binding assay for rapid detection of healthcare-associated infections.Scientific reports · 2026Article
- High prevalence of Q fever among patients clinically diagnosed with brucellosis in a Kenyan pastoralist community.Frontiers in epidemiology · 2026Article
- Evaluation of Brucella IgM and IgG ELISA Responses in Relation to Blood Culture Positivity in Pediatric Brucellosis.Infection and drug resistance · 2026Article
- Serological diagnosis of human brucellosis in Morocco and prospects for advanced diagnostic techniques.Iranian journal of microbiology · 2025Article
- Comparative Evaluation of Diagnostic Tests for Brucellosis in Humans and Animals: A Meta-Analytical Approach.Veterinary sciences · 2025Article
- An ultra-sensitive and point-of-care time-resolved fluorescence immunochromatographic platform for trace-level brucellosis antibody rapid on-site detection.Journal of nanobiotechnology · 2025Article
- Establishment of an I-ELISA method based on multi-epitope fusion protein for diagnosis of human brucellosis.PLoS neglected tropical diseases · 2025Article
- Case report: diagnosis of neurobrucellosis in a non-endemic area child using metagenomic next-generation sequencing.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2025Article
- Unraveling brucellosis: advances in pathogenesis, diagnostic strategies, therapeutic innovations, and public health perspectives.Frontiers in medicine · 2025Review
- Sero-prevalence of human brucellosis and associated factors among febrile patients attending Moyale Primary Hospital, Southern Ethiopia, 2023: Evidences from pastoralist community.PLoS neglected tropical diseases · 2024Article
- Clinical characterization of brucellosis in children from non-pastoral areas: a report of five cases.BMC infectious diseases · 2024Article
- Seroprevalence of brucellosis among high-risk individuals in Madinah, Saudi Arabia.Veterinary world · 2024Article
- Determining Diagnostic Sensitivity: A Comparison of Rose Bengal Test, Coombs Gel Test, ELISA and Bacterial Culture in Brucellosis Diagnosis-Analyzing Clinical Effectiveness in Light of Inflammatory Markers.Diagnostics (Basel, Switzerland) · 2024Article
- Probing the expression and adhesion of glycans involved in Helicobacter pylori infection.Scientific reports · 2024Article
- Sero-prevalence and risk factors associated with occurrence of anti-Brucella antibodies among slaughterhouse workers in Uganda.PLoS neglected tropical diseases · 2024Article
- DNA Sensor for the Detection ofInternational journal of molecular sciences · 2023Article
- Laboratory diagnostics for primary spinal infections in pediatric and adult populations: a narrative review.North American Spine Society journal · 2023Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundBrucellosis is endemic in many areas in China. The current diagnosis of Brucellosis predominantly relies on the traditional bacterial culture and serum agglutination test. In this study, we aimed to explore the value of ELISA in the diagnosis of Brucellosis in Chinese population.
methodsWe recruited 235 patients with a diagnosis of Brucellosis at different clinical stages: 117 in acute, 78 in subacute, and 40 in chronic. We also recruited 248 control patients who presented with similar clinical symptoms but with a different diagnosis other than Brucellosis. In addition, 90 healthy volunteers were also recruited. Bacterial culture, agglutination test and ELISA assay were performed to detect Brucella spp.
resultsAmong 235 patients with Brucellosis, 51 (21.7%) was positive for bacterial culture, 150 (63.8%) were positive by agglutination test, and 232 (98.7%) were positive by ELISA (IgG and/or IgM). When we stratified the patients based on the disease stages (acute, subacute and chronic), ELISA was the most sensitive method and showed a highest positive rate in all stages. By Receiver Operating Characteristic Curve analysis of ELISA results, we found that measurement of IgG level was superior to measurement of IgM level (AUC, 0.993 versus 0.877). Since the measurement of IgG itself missed rare cases in acute phase, we recommended measuring IgG and IgM simultaneously by ELISA for the diagnosis of Brucellosis. In term of the specificity of ELISA in the diagnosis of Brucellosis, our study showed that only 1.6% (4/248) non-Brucellosis patients were positive by ELISA; all positive cases were IgM only and none showed positive IgG. Similar results were found in healthy volunteers. In summary, our study concluded that ELISA is the most sensitive and specific method to detect Brucellosis in Chinese population.
conclusionsELISA assay is sensitive, fast, and convenient to detect Brucellosis. It shows the high sensitivity and specifity and should be used as a routine lab test when Brucellosis is suspected in clinical practice.
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