Evidence map›Paper›PMID 31906861›Full record

ArticleBMC genomics2020

Preliminary investigation demonstrating the GHITM gene probably involved in apoptosis and growth of the golden apple snail (Pomacea canaliculata).

Wenchao Yu, Baolu Zhang, Hongce Song, Rui Zhan, Lingling Li, Cheng He, Qiuyun Jiang, Xiaona Wang, Lei Wei, Nannan Zhao and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.5field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 14 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 1 country.

Wenchao YuSchool of Agriculture, Ludong University, Yantai, 264025, China.
Baolu ZhangOceanic Consultation Center, Ministry of Natural Resources of the People's Republic of China, Beijing, 100071, China.
Hongce SongSchool of Agriculture, Ludong University, Yantai, 264025, China.
Rui ZhanSchool of Agriculture, Ludong University, Yantai, 264025, China.
Lingling LiSchool of Agriculture, Ludong University, Yantai, 264025, China.
Cheng HeSchool of Agriculture, Ludong University, Yantai, 264025, China.
Qiuyun JiangSchool of Agriculture, Ludong University, Yantai, 264025, China.
Xiaona WangSchool of Agriculture, Ludong University, Yantai, 264025, China.
Lei WeiSchool of Agriculture, Ludong University, Yantai, 264025, China.
Nannan ZhaoKey Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture), Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 5Yushan Road, Qingdao, 266003, Shandong, China.
Wen GuoCenter for Mollusc Study and Development, Marine Biology Institute of Shandong Province, Qingdao, 266104, China.
Xiaotong WangSchool of Agriculture, Ludong University, Yantai, 264025, China. wangxiaotong999@163.com.
Ludong University · CNMarine Biology Institute of Shandong Province · CNMinistry of Water Resources of the People's Republic of China · CNOcean University of China · CN

Funding

Fine Agricultural Breeds Project of Shandong Province, China 2019LZGC020Key R&D Program of Yantai City, China 2017ZH054Key Technology Research and Development Program of Shandong (CN) 2018GHY115027Modern Agricultural Industry Technology System of Shandong Province, China SDAIT-14-03National Key R$D Program of China 2018YFD0901400National Natural Science Foundation of China 41876193National Natural Science Foundation of China 41906088Natural Science Foundation of Shandong Province ZR2019MC002Plan of Excellent Youth Innovation Team of Colleges and universities in Shandong Province, China 2019KJF004Special Funds for Taishan Scholars Project of Shandong Province, China tsqn201812094
6 · The paper itself

Abstract

backgroundGrowth hormone inducible transmembrane protein (GHITM) is a highly conserved transmembrane protein. This study was conducted to investigate the role of GHITM gene in the apoptosis and growth of the golden apple snail Pomacea canaliculate.

resultsThe complete cDNA of this gene was cloned using the rapid amplification of cDNA ends (RACE) method and subjected to bioinformatics analysis. The full-length cDNA was 2242 bp, including an open reading frame of 1021 bp that encoded a protein of 342 amino acid residues. The mRNA expression profiles of GHITM gene in different tissues (liver, kidney, gonad and foot) and different growth phases (6-months old and 2-years old) showed that it was expressed in various tissues and different growth phases. Silencing of the GHITM gene by RNAi (RNA interference) experiments revealed that the GHITM gene possibly plays a role in inhibiting apoptosis through detecting the Caspase (Cysteine-requiring Aspartate Protease)-3 activity. In addition, the aperture width and body whorl length of the snail was significantly affected by RNAi, suggesting that this gene plays a significant role in promoting the growth of the organism.

conclusionsThese results demonstrated that the GHITM gene was involved in apoptosis and growth in golden apple snail.

Indexed as

Amino Acid SequenceAnimalsApoptosisBase SequenceCloning, MolecularDNA, ComplementaryGene Expression ProfilingMembrane ProteinsOpen Reading FramesPhylogenyRNA InterferenceSnailsDNA, ComplementaryMembrane ProteinsCell apoptosisComplete cDNAGHITM geneGrowthPomacea canaliculateRNAi

Identifiers

PMID31906861
PMCPMC6945724
OpenAlexW2999178667

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.