Evidence map›Paper›PMID 31906856›Full record

ArticleBMC plant biology2020

Cytological observation of anther structure and genetic investigation of a thermo-sensitive genic male sterile line 373S in Brassica napus L.

Yanyan Sun, Dongsuo Zhang, Zhenzhen Wang, Yuan Guo, Xiaomin Sun, Wei Li, Wenliang Zhi, Shengwu Hu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC plant biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 9 citations in OpenAlex.

  1. Genome-Wide Identification of the SPP/SPPL Gene Family andInternational journal of molecular sciences · 2024
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Yanyan SunState Key Laboratory of Crop Stress Biology in Arid Areas and College of Agronomy, Northwest A&F University, Agri-Science Building Rm 733, Yangling, 712100, Shaanxi, China.
Dongsuo ZhangState Key Laboratory of Crop Stress Biology in Arid Areas and College of Agronomy, Northwest A&F University, Agri-Science Building Rm 733, Yangling, 712100, Shaanxi, China.
Zhenzhen WangState Key Laboratory of Crop Stress Biology in Arid Areas and College of Agronomy, Northwest A&F University, Agri-Science Building Rm 733, Yangling, 712100, Shaanxi, China.
Yuan GuoState Key Laboratory of Crop Stress Biology in Arid Areas and College of Agronomy, Northwest A&F University, Agri-Science Building Rm 733, Yangling, 712100, Shaanxi, China.
Xiaomin SunState Key Laboratory of Crop Stress Biology in Arid Areas and College of Agronomy, Northwest A&F University, Agri-Science Building Rm 733, Yangling, 712100, Shaanxi, China.
Wei LiState Key Laboratory of Crop Stress Biology in Arid Areas and College of Agronomy, Northwest A&F University, Agri-Science Building Rm 733, Yangling, 712100, Shaanxi, China.
Wenliang ZhiState Key Laboratory of Crop Stress Biology in Arid Areas and College of Agronomy, Northwest A&F University, Agri-Science Building Rm 733, Yangling, 712100, Shaanxi, China.
Shengwu HuState Key Laboratory of Crop Stress Biology in Arid Areas and College of Agronomy, Northwest A&F University, Agri-Science Building Rm 733, Yangling, 712100, Shaanxi, China. swhu83251@nwsuaf.edu.cn.ORCID https://orcid.org/0000-0001-6859-6873
Northwest A&F University · CNNorth West Agriculture and Forestry University · CNShaanxi University of Technology · CN

Funding

Modern Crop Seed Industry Project of Shaanxi Province 20171010000004the Key Research and Development Project in Shaanxi Province of China 2018ZDXM-NY-008
6 · The paper itself

Abstract

backgroundPhotoperiod and/or thermo-sensitive male sterility is an effective pollination control system in crop two-line hybrid breeding. We previously discovered the spontaneous mutation of a partially male sterile plant and developed a thermo-sensitive genic male sterile (TGMS) line 373S in Brassica napus L. The present study characterized this TGMS line through cytological observation, photoperiod/ temperature treatments, and genetic investigation.

resultsMicroscopic observation revealed that the condensed cytoplasm and irregular exine of microspores and the abnormal degradation of tapetum are related to pollen abortion. Different temperature and photoperiod treatments in field and growth cabinet conditions indicated that the fertility alteration of 373S was mainly caused by temperature changes. The effects of photoperiod and interaction between temperature and photoperiod were insignificant. The critical temperature leading to fertility alteration ranged from 10 °C (15 °C/5 °C) to 12 °C (17 °C/7 °C), and the temperature-responding stage was coincident with anther development from pollen mother cell formation to meiosis stages. Genetic analysis indicated that the TGMS trait in 373S was controlled by one pair of genes, with male sterility as the recessive. Multiplex PCR analysis revealed that the cytoplasm of 373S is pol type.

conclusionsOur study suggested that the 373S line in B. napus has a novel thermo-sensitive gene Bnms

Indexed as

Brassica napusFlowersGenes, PlantPhotoperiodPlant InfertilityPollenTemperatureBrassica napus L.InheritanceMicroscopic observationThermo-sensitive genic male sterility

Identifiers

PMID31906856
PMCPMC6945434
OpenAlexW2999629034

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.