ArticlePhotochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology2020
Raf-like kinases CBC1 and CBC2 negatively regulate stomatal opening by negatively regulating plasma membrane H
Article in Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 23 citations in OpenAlex.
- Guard cell signal transduction mechanisms controlling stomatal movements by light, abscisic acid, CO2, VPD and heat.Plant physiology · 2026Review
- LRS1 phosphorylated in response to blue light regulates stomatal opening.Journal of plant research · 2026Article
- The red and blue light dialog of stomatal regulation for water-efficient crops.Plant physiology · 2026Review
- COPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
- Subfamily C7 Raf-like kinases MRK1, RAF26, and RAF39 regulate immune homeostasis and stomatal opening in Arabidopsis thaliana.The New phytologist · 2024Article
- Starch metabolism in guard cells: At the intersection of environmental stimuli and stomatal movement.Plant physiology · 2024Review
- Stomatal CO2 responses at sub- and above-ambient CO2 levels employ different pathways in Arabidopsis.Plant physiology · 2024Article
- A network-based modeling framework reveals the core signal transduction network underlying high carbon dioxide-induced stomatal closure in guard cells.PLoS biology · 2024Article
- Stomatal responses to VPD utilize guard cell intracellular signaling components.Frontiers in plant science · 2024Article
- Elevated COThe New phytologist · 2022Article
- A tonoplast-localized magnesium transporter is crucial for stomatal opening in Arabidopsis under high MgThe New phytologist · 2022Article
- The maize single-nucleus transcriptome comprehensively describes signaling networks governing movement and development of grass stomata.The Plant cell · 2022Article
- The Raf-like kinase Raf36 negatively regulates plant resistance against the oomycete pathogen Phytophthora parasitica by targeting MKK2.Molecular plant pathology · 2022Article
- Proton and calcium pumping P-type ATPases and their regulation of plant responses to the environment.Plant physiology · 2021Review
- CIPK23 regulates blue light-dependent stomatal opening in Arabidopsis thaliana.The Plant journal : for cell and molecular biology · 2020Article
- Light-Mediated Signaling and Metabolic Changes Coordinate Stomatal Opening and Closure.Frontiers in plant science · 2020Review
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stomatal pores, which are surrounded by pairs of guard cells in the plant epidermis, regulate gas exchange between plants and the atmosphere, thereby controlling photosynthesis and transpiration. Blue light works as a signal to guard cells, to induce intracellular signaling and open stomata. Blue light receptor phototropins (phots) are activated by blue light; phot-mediated signals promote plasma membrane (PM) H+-ATPase activity via C-terminal Thr phosphorylation, serving as the driving force for stomatal opening in guard cells. However, the details of this signaling process are not fully understood. In this study, through an in vitro screening of phot-interacting protein kinases, we obtained the CBC1 and CBC2 that had been reported as signal transducers in stomatal opening. Promoter activities of CBC1 and CBC2 indicated that both genes were expressed in guard cells. Single and double knockout mutants of CBC1 and CBC2 showed no lesions in the context of phot-mediated phototropism, chloroplast movement, or leaf flattening. In contrast, the cbc1cbc2 double mutant showed larger stomatal opening under both dark and blue light conditions. Interestingly, the level of phosphorylation of C-terminal Thr of PM H+-ATPase was higher in double mutant guard cells. The larger stomatal openings of the double mutant were effectively suppressed by the phytohormone abscisic acid (ABA). CBC1 and CBC2 interacted with BLUS1 and PM H+-ATPase in vitro. From these results, we conclude that CBC1 and CBC2 act as negative regulators of stomatal opening, probably via inhibition of PM H+-ATPase activity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.