ArticleJournal of experimental botany2020
Length-dependent accumulation of double-stranded RNAs in plastids affects RNA interference efficiency in the Colorado potato beetle.
Article in Journal of experimental botany, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed, 73 citations in OpenAlex.
- Optimization of dsRNA Length and Target Position for Enhanced RNA Interference Efficiency Targeting the Chitinase GeneInsects · 2026Article
- RNAi: the next wave of green agriculture, challenges, and bridging the translational gap.Plant cell reports · 2026Review
- Emerging experimental and bioinformatic approaches in RNA interference-based pest control research.Insect molecular biology · 2026Review
- Development of chloroplast transformation for five species in the genus Nicotiana.The Plant journal : for cell and molecular biology · 2025Article
- Recent advances in understanding of the mechanisms of RNA interference in insects.Insect molecular biology · 2025Review
- RNAi Strategies Against Downy Mildews: Insights Into dsRNA Uptake and Silencing.Molecular plant pathology · 2025Article
- Environmental factors affecting RNAi efficacy: Temperature but not plant cultivar influences Colorado potato beetle's response to insecticidal dsRNA.Insect molecular biology · 2025Article
- Review
- Optimizing dsRNA sequences for RNAi in pest control and research with the dsRIP web platform.BMC biology · 2025Article
- SilencingInsects · 2024Article
- Host-Delivered RNA Interference for Durable Pest Resistance in Plants: Advanced Methods, Challenges, and Applications.Molecular biotechnology · 2024Review
- Control of two insect pests by expression of a mismatch corrected double-stranded RNA in plants.Plant biotechnology journal · 2024Article
- Review
- Odorant binding protein 18 increases the pathogen resistance of the imported willow leaf beetle,Frontiers in cellular and infection microbiology · 2024Article
- Engineering of insecticidal hybrid gene into potato chloroplast genome exhibits promising control of Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae).Transgenic research · 2023Article
- Inefficient uptake of small interfering RNAs is responsible for their inability to trigger RNA interference in Colorado potato beetle cells.Archives of insect biochemistry and physiology · 2023Article
- Transgenic Microalgae Expressing Double-Stranded RNA as Potential Feed Supplements for Controlling White Spot Syndrome in Shrimp Aquaculture.Microorganisms · 2023Article
- The Study of Cell-Penetrating Peptides to Deliver dsRNA and siRNA by Feeding in the Desert Locust,Insects · 2023Article
- New Insights on the Integrated Management of Plant Diseases by RNA Strategies: Mycoviruses and RNA Interference.International journal of molecular sciences · 2022Review
- Efficient control of western flower thrips by plastid-mediated RNA interference.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transplastomic potato plants expressing double-stranded RNA (dsRNA) targeted against essential genes of the Colorado potato beetle (CPB) can be lethal to larvae by triggering an RNA interference (RNAi) response. High accumulation levels of dsRNAs in plastids are crucial to confer an efficient RNAi response in the insects. However, whether length and sequence of the dsRNA determine the efficacy of RNAi and/or influence the level of dsRNA accumulation in plastids is not known. We compared the RNAi efficacy of different lengths of dsRNA targeted against the CPB β-Actin gene (ACT) by feeding in vitro-synthesized dsRNAs to larvae. We showed that, while the 60 bp dsRNA induced only a relatively low RNAi response in CPB, dsRNAs of 200 bp and longer caused high mortality and similar larval growth retardation. When the dsRNAs were expressed from the plastid (chloroplast) genome of potato plants, we found that their accumulation were negatively correlated with length. The level of dsRNA accumulation was positively associated with the observed mortality, suppression of larval growth, and suppression of target gene expression. Importantly, transplastomic potato plants expressing the 200 bp dsRNA were better protected from CPB than plants expressing the 297 bp dsRNA, the best-performing line in our previous study. Our results suggest that the length of dsRNAs is an important factor that influences their accumulation in plastids and thus determines the strength of the insecticidal RNAi effect. Our findings will aid the design of optimized dsRNA expression constructs for plant protection by plastid-mediated RNAi.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.