ArticleThe Journal of physical chemistry1994
Light Quenching of Fluorescence Using Time-Delayed Laser Pulses As Observed by Frequency-Domain Fluorometry.
Article in The Journal of physical chemistry, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Fluorescence lifetime characterization of novel low-pH probes.Analytical biochemistry · 2001Article
- Definition and properties of the emission anisotropy in the absence of cylindrical symmetry of the emission field: Application to the light quenching experiments.The Journal of chemical physics · 1999Article
- Anisotropy Spectra of the Solvent-Sensitive Fluorophore 4-Dimethylamino-4'-Cyanostilbene in the Presence of Light QuenchingJournal of fluorescence · 1998Article
- Effects of Light Quenching on the Emission Spectra and Intensity Decays of Fluorophore Mixtures.Journal of fluorescence · 1997Article
- Light quenching of pyridine2 fluorescence with time-delayed pulses.Biophysical chemistry · 1997Article
- Wavelength-selective light quenching of biochemical fluorophores.Journal of biomedical optics · 1997Article
- Fluorescence Spectral Properties of 2,5-Diphenyl-1,3,4-oxadiazole with Two-Color Two-Photon Excitation.The Journal of physical chemistry · 1996Article
- Effect of Fluorescence Quenching by Stimulated Emission on the Spectral Properties of a Solvent-Sensitive FluorophoreThe Journal of physical chemistry · 1996Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
We describe experimental observations of fluorescence quenching by time-delayed light pulses whose wavelength overlaps the emission spectrum of 4-(dimethylamino)-4'-cyanostilbene (DCS). The relative cross sections for light quenching were proportional to the amplitude of the emission spectra at the light quenching wavelength. The frequency-domain intensity and anisotropy decay measurements showed oscillations resulting from time-delayed light quenching. The amplitude of the oscillations depends upon the amount of light quenching. The frequency of the oscillations depends upon the delay between the excitation and quenching pulses. To the best of our knowledge, only a stepwise decrease in the intensity or anisotropy could produce such oscillations in the frequency-domain data. Light quenching of fluorescence is thus shown to provide a means to control the number and orientation of the excited fluorophores. The use of multiple light pulses for excitation and quenching can have far-reaching applications in the use of time-resolved fluorescence in physical chemistry and biophysics.
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Registered trials
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