Evidence map›Paper›PMID 31902496›Full record

ArticleVeterinary microbiology2020

A virome sequencing approach to feline oral squamous cell carcinoma to evaluate viral causative factors.

Shirley Chu, Todd N Wylie, Kristine M Wylie, Gayle C Johnson, Zachary L Skidmore, Maren Fleer, Obi L Griffith, Jeffrey N Bryan

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Veterinary microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
2.9field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 37 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Feline Papillomatosis.Viruses · 2025
    Review
  5. Review
  6. Review
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Feline oral squamous cell carcinoma andFrontiers in veterinary science · 2023
    Article
  13. Article
  14. Review
  15. Review
  16. Review
  17. Review
  18. Article
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Shirley ChuDepartment of Veterinary Medicine and Surgery, University of Missouri, 900 E. Campus Drive, Columbia, MO 65211, USA. Electronic address: chus@missouri.edu.
Todd N WylieMcDonnell Genome Institute, Washington University, 4444 Forest Park Ave, St. Louis, MO 63108, USA.
Kristine M WylieMcDonnell Genome Institute, Washington University, 4444 Forest Park Ave, St. Louis, MO 63108, USA.
Gayle C JohnsonDepartment of Veterinary Pathobiology, University of Missouri, 800 E. Campus Loop, Columbia, MO 65211, USA.
Zachary L SkidmoreMcDonnell Genome Institute, Washington University, 4444 Forest Park Ave, St. Louis, MO 63108, USA.
Maren FleerDepartment of Veterinary Medicine and Surgery, University of Missouri, 900 E. Campus Drive, Columbia, MO 65211, USA.
Obi L GriffithMcDonnell Genome Institute, Washington University, 4444 Forest Park Ave, St. Louis, MO 63108, USA.
Jeffrey N BryanDepartment of Veterinary Medicine and Surgery, University of Missouri, 900 E. Campus Drive, Columbia, MO 65211, USA.
James S. McDonnell Foundation · USUniversity of Missouri · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Feline oral squamous cell carcinoma (FOSCC) may be the best naturally-occurring model of human head and neck squamous cell carcinoma (HNSCC). HNSCC can be broadly divided into human papillomavirus (HPV)-negative cancers and HPV-positive cancers where HPV is the causative agent. Previous studies in FOSCC have used both species-specific and species-nonspecific PCR primers that may be insensitive to the detection of PVs and other viruses that may be divergent from known sequences. ViroCap is a targeted capture and next generation sequencing tool that was designed to identify all known vertebrate DNA and RNA viruses. In this study we used a metagenomic approach using ViroCap for DNA viruses in 20 FOSCC, 9 normal feline oral mucosal, and 8 suspected PV positive control samples. We tested the hypothesis that viruses would be enriched in FOSCC compared to normal oral mucosa. The virome of the FOSCC and normal feline oral mucosa consisted of feline foamy virus in 7/20 and 2/9 (35% and 22%), feline torque teno virus in 2/20 and 0/9 (10% and 0%), alphaherpesvirus in 2/10 and 0/9 (10% and 0%), FIV (0% and 22%), Epstein-Barr virus in 1/20 and 0/9 (5% and 0%) and feline papillomavirus in 1/20 and 0/9 samples (5% and 0% respectively). Felis catus papillomavirus-3 was found in 1 of 20 FOSCC samples. A virus was not associated consistently with FOSCC. If PVs have a role in FOSCC it is at most a supplementary or uncommon role. FOSCC appears most closely related to HPV-negative HNSCC. Future research on FOSCC should focus on identifying genetic and environmental causes.

Indexed as

AnimalsCarcinoma, Squamous CellCatsCoinfectionDNA, ViralFemaleHigh-Throughput Nucleotide SequencingMaleMouth NeoplasmsPapillomavirus InfectionsParaffin EmbeddingVirusesDNA, ViralEpstein Barr virusFeline oral squamous cell carcinomaMetagenomicSequencingVirome

Identifiers

PMID31902496
PMCPMC7117531
OpenAlexW2983908670

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.