ArticlePlant molecular biology2020
Full-length transcriptome analysis of Coptis deltoidea and identification of putative genes involved in benzylisoquinoline alkaloids biosynthesis based on combined sequencing platforms.
Article in Plant molecular biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 59 citations in OpenAlex.
- Functional Characterization of Two Methyltransferases Involved in Benzylisoquinoline Alkaloid Biosynthesis in Sinomenium acutum.Chemistry & biodiversity · 2026Article
- Transcriptomic and Metabolomic Insights into Benzylisoquinoline Alkaloid Biosynthesis in Goldthread (International journal of molecular sciences · 2025Article
- Application and Progress of Genomics in Deciphering the Genetic Regulation Mechanisms of Plant Secondary Metabolites.Plants (Basel, Switzerland) · 2025Review
- Full-length transcriptome profiling ofPeerJ · 2025Article
- Transcriptome sequencing of medical herb Salvia Rosmarinus (Rosemary) revealed the phenylpropanoid biosynthesis pathway genes and their phylogenetic relationships.Molecular biology reports · 2024Article
- Designing combinational herbal drugs based on target space analysis.BMC complementary medicine and therapies · 2024Article
- Integrative analysis of the metabolome and transcriptome reveals the mechanism of polyphenol biosynthesis inFrontiers in plant science · 2024Article
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- Applications of some advanced sequencing, analytical, and computational approaches in medicinal plant research: a review.Molecular biology reports · 2023Review
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- Integrating Full-Length and Second-Generation Transcriptomics to Reveal Differentially Expressed Genes Associated with the Development ofLife (Basel, Switzerland) · 2023Article
- Histology, physiology, and transcriptomic and metabolomic profiling reveal the developmental dynamics of annual shoots in tree peonies (Horticulture research · 2023Article
- Full-length transcriptome combined with RNA sequence analysis of Fraxinus chinensis.Genes & genomics · 2023Article
- Isolation and Characterization of the Genes Involved in the Berberine Synthesis Pathway in Asian Blue Cohosh,Plants (Basel, Switzerland) · 2023Article
- Full-length transcriptome, proteomics and metabolite analysis reveal candidate genes involved triterpenoid saponin biosynthesis inFrontiers in plant science · 2023Article
- Comparative Transcriptomics for Genes Related to Berberine and Berbamine Biosynthesis in Berberidaceae.Plants (Basel, Switzerland) · 2022Article
- Transcriptome Analysis ofFrontiers in plant science · 2022Article
- Full-length transcriptome and metabolite analysis reveal reticuline epimerase-independent pathways for benzylisoquinoline alkaloids biosynthesis inFrontiers in plant science · 2022Article
- Characterization of the anti-AChE potential and alkaloids in Rhizoma Coptidis from differentFrontiers in plant science · 2022Article
- Separation and purification of plant terpenoids from biotransformation.Engineering in life sciences · 2021Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
key messageThe study carry out comprehensive transcriptome analysis of C. deltoidea and exploration of BIAs biosynthesis and accumulation based on UHPLC-MS/MS and combined sequencing platforms. Coptis deltoidea is an important medicinal plant with a long history of medicinal use, which is rich in benzylisoquinoline alkaloids (BIAs). In this study, Ultra performance liquid chromatography-electrospray ionization tandem mass spectrometry (UHPLC-ESI-MS/MS) and combined sequencing platforms were performed for exploration of BIAs biosynthesis, accumulation and comprehensive transcriptome analysis of C. deltoidea. By metabolism profiling, the accumulation of ten BIAs was analyzed using UHPLC-MS/MS and different contents were observed in different organs. From transcriptome sequencing result, we applied single-molecule real-time (SMRT) sequencing to C. deltoidea and generated a total of 75,438 full-length transcripts. We proposed the candidate biosynthetic pathway of tyrosine, precursor of BIAs, and identified 64 full length-transcripts encoding enzymes putatively involved in BIAs biosynthesis. RNA-Seq data indicated that the majority of genes exhibited relatively high expression level in roots. Transport of BIAs was also important for their accumulation. Here, 9 ABC transporters and 2 MATE transporters highly homologous to known alkaloid transporters related with BIAs transport in roots and rhizomes were identified. These findings based on the combined sequencing platforms provide valuable genetic information for C. deltoidea and the results of transcriptome combined with metabolome analysis can help us better understand BIAs biosynthesis and transport in this medicinal plant. The information will be critical for further characterization of C. deltoidea transcriptome and molecular-assisted breeding for this medicinal plant with scarce resources.
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