ArticleCell and tissue research2020
A reduction of primary cilia but not hedgehog signaling disrupts morphogenesis in testicular organoids.
Article in Cell and tissue research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 21 citations in OpenAlex.
- [Research progress on the roles of primary cilia in fertility].Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2025Review
- New Insights into the Molecular Actions of Grosheimin, Costunolide, and α- and β-Cyclocostunolide on Primary Cilia Structure and Hedgehog Signaling.International journal of molecular sciences · 2025Article
- Platelet-derived growth factor signaling in porcine testicular tubular somatic cells.Cell and tissue research · 2025Article
- Human efferent ductules and epididymis display unique cell lineages with motile and primary cilia.Andrology · 2025Article
- Generation of Organotypic Testicular Organoids from Rat and Pig Primary Cells in Microwell Culture.Methods in molecular biology (Clifton, N.J.) · 2025Article
- The role of primary cilia in the testis.Current opinion in endocrine and metabolic research · 2024Article
- From mesenchymal niches to engineeredMaterials today. Bio · 2022Article
- Generation and Characterization of Bovine Testicular Organoids Derived from Primary Somatic Cell Populations.Animals : an open access journal from MDPI · 2022Article
- A brief history of testicular organoids: from theory to the wards.Journal of assisted reproduction and genetics · 2022Review
- Bioengineering Approaches for the Advanced Organoid Research.Advanced materials (Deerfield Beach, Fla.) · 2021Review
- Regulation of Cell Types Within Testicular Organoids.Endocrinology · 2021Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Most mammalian cells possess a single, non-motile primary cilium that plays an important role in mediating cellular signaling pathways, such as Hedgehog (Hh) signaling. Primary cilia are present on testicular somatic cells and demonstrate a temporal expression during development; however, their role in testicular morphogenesis is not well characterized. To investigate the role of primary cilia and Hh signaling in Sertoli cells on morphogenesis, we inhibited assembly of primary cilia through CRISPR Cas9-mediated gene editing of ODF2, a structural component of primary cilia and siRNA-mediated gene silencing of IFT88, a functional component of the intraflagellar transport system. Knockdown of ODF2 and IFT88 resulted in a 50% reduction in the number of cells with primary cilia and significant shortening of the remaining cilia. The expression of GLI1, a downstream target of Hh signaling, was significantly reduced when IFT88 but not ODF2, was downregulated. When morphogenesis was examined using tubule formation in vitro and a novel testicular organoid system, loss of cilia after knockdown of both targets affected cellular assembly and organization. While the Hh pathway was found to be active during morphogenesis in vitro, addition of the Hh antagonist cyclopamine did not affect morphogenesis in either in vitro system. These results indicate that primary cilia are important for morphogenesis in vitro but Hh signaling is not the cilia-mediated pathway responsible for orchestrating morphogenic organization.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.