ArticleNature communications2020
Conditional control of RNA-guided nucleic acid cleavage and gene editing.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 96 citations in OpenAlex.
- Non-Viral CRISPR carriers: transient delivery with lasting effects.Drug delivery · 2026Review
- Glutathione-Responsive Acyl-Modifications for Targeted RNA Decaging and Prolonged Protein Synthesis.Angewandte Chemie (International ed. in English) · 2026Article
- Palladium-triggered bioorthogonal phenylalanine decaging.Nature chemistry · 2026Article
- Reversible one-step acylation facilitates mitochondrial delivery of functional RNA.Chemical science · 2026Article
- Fluorogenic Covalent Probes for RNA.Journal of the American Chemical Society · 2025Article
- CRISPR-based Transcriptional Regulation: Technologies, Applications, and Future Directions.DNA · 2025Article
- Conditional Control of CRISPR/Cas9 Function by Chemically Modified Oligonucleotides.Molecules (Basel, Switzerland) · 2025Review
- Tailoring and reversing m6A editing with sequential RNA bioorthogonal chemistry.Nucleic acids research · 2025Article
- Strategic base modifications refine RNA function and reduce CRISPR-Cas9 off-targets.Nucleic acids research · 2025Article
- Reversible RNA Acylation Using Bio-Orthogonal Chemistry Enables Temporal Control of CRISPR-Cas9 Nuclease Activity.ACS chemical biology · 2024Article
- Selective Arylation of RNA 2'-OH Groups via SAngewandte Chemie (International ed. in English) · 2024Article
- Second-Generation Chiral Amino Acid Derivatives Afford High Stereoselectivity and Stability in Aqueous RNA Acylation.The Journal of organic chemistry · 2024Article
- RNA Control via Redox-Responsive Acylation.Angewandte Chemie (International ed. in English) · 2024Article
- 2'-OH as a universal handle for studying intracellular RNAs.Cell chemical biology · 2024Review
- Designing Epigenome Editors: Considerations of Biochemical and Locus Specificities.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Efficient post-synthesis incorporation and conjugation of reactive ketones in RNAChemical communications (Cambridge, England) · 2023Article
- Stereoselective RNA reaction with chiral 2'-OH acylating agents.Chemical science · 2023Article
- Reactive oxygen signaling molecule inducible regulation of CRISPR-Cas9 gene editing.Cell biology and toxicology · 2023Article
- Reversible 2'-OH acylation enhances RNA stability.Nature chemistry · 2023Article
- Regulation of the CRISPR-Cas12a system by methylation and demethylation of guide RNA.Chemical science · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Prokaryotes use repetitive genomic elements termed CRISPR (clustered regularly interspaced short palindromic repeats) to destroy invading genetic molecules. Although CRISPR systems have been widely used in DNA and RNA technology, certain adverse effects do occur. For example, constitutively active CRISPR systems may lead to a certain risk of off-target effects. Here, we introduce post-synthetic masking and chemical activation of guide RNA (gRNA) to controlling CRISPR systems. An RNA structure profiling probe (2-azidomethylnicotinic acid imidazolide) is used. Moreover, we accomplish conditional control of gene editing in live cells. This proof-of-concept study demonstrates promising potential of chemical activation of gRNAs as a versatile tool for chemical biology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.