ArticleExperimental & applied acarology2020
A survey of proteins in midgut contents of the tick, Haemaphysalis flava, by proteome and transcriptome analysis.
Article in Experimental & applied acarology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 9 citations in OpenAlex.
- Characterization and expression analysis of heat shock proteins HfHspc1 and HfHspc4 in Haemaphysalis flava ticks.Parasitology research · 2024Article
- The proteomic content of Varroa destructor gut varies according to the developmental stage of its host.PLoS pathogens · 2024Article
- Egg Protein Compositions over Embryonic Development inAnimals : an open access journal from MDPI · 2024Article
- Tick Immunobiology and Extracellular Traps: An Integrative Vision to Control of Vectors.Pathogens (Basel, Switzerland) · 2021Review
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Authors and funding
2 authors at 1 institution in 1 country.
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Abstract
Tick blood meals are stored and digested in their midguts. Blood digestion is complex, and many proteins are involved. Study of the tick-derived proteins in the midgut content may aid in the discovery of active molecules that would be useful for anti-tick vaccines. We analyzed the midgut content proteomes of partially engorged female Haemaphysalis flava, fully engorged female H. flava, and hedgehog serum using liquid chromatography tandem-mass spectrometry and label-free quantitation. In this study, high-confidence protein profiling of tick midgut content was determined. Based on the search against our in-house transcriptome database, the 28 high-confidence proteins were identified. Of these, 17 were identified as tick-derived, and the rest were of unspecified origin (proteins that could not be differentiated as host-derived or tick-derived proteins). The function of these midgut content proteins identified here may involve nutrient transportation, anti-coagulation, erythrocyte lysis, detoxification, lipid metabolism, and immunization. The presence of hemoglobin suggested that the red blood cells were lysed in the gut lumen. The midgut contents contain a large amount of fibrinogen and it has the ability to clot immediately. The midgut contained mostly host-derived proteins, and these host proteins provide rich nutrients for tick development and reproduction. However, some intracellular proteins were also identified, suggesting the possibility of shedding of the midgut epithelium and ingestion of saliva during feeding. This finding advances our understanding of the digestive mechanism and will be useful in the screening of vaccine antigens.
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