ArticlePlant molecular biology2020
A novel basic helix-loop-helix transcription factor, ZjICE2 from Zoysia japonica confers abiotic stress tolerance to transgenic plants via activating the DREB/CBF regulon and enhancing ROS scavenging.
Article in Plant molecular biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 48 citations in OpenAlex.
- Abscisic Acid-Mediated Drought Tolerance inCurrent issues in molecular biology · 2026Review
- Genome-wide identification and cold stress expression of inducer of CBF expression (ICE) transcription factors in Taraxacum kok-saghyz.Functional & integrative genomics · 2026Article
- Overexpression of TkMYC2 enhances drought tolerance and rubber-producing capacity in transgenic Taraxacum kok-saghyz.Planta · 2026Article
- Integrative Physiological, Metabolomic and Transcriptomic Analyses Uncover the Mechanisms Underlying Differential Responses of TwoBiomolecules · 2025Article
- Genetic and genomic resources for turfgrasses: status, applications, and prospects.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Review
- Upregulated expression of MYB4, DREB1 and AP37 transcription factors modulates cold stress response in high-altitude Himalayan rice via time-dependent ROS regulation.Molecular biology reports · 2025Article
- Plant Coping with Cold Stress: Molecular and Physiological Adaptive Mechanisms with Future Perspectives.Cells · 2025Review
- Genome-wide identification of bHLH transcription factors and functional analysis in salt gland development of the recretohalophyte sea lavender (Horticulture research · 2024Article
- RcbHLH59-RcPRs module enhances salinity stress tolerance by balancing NaHorticulture research · 2023Article
- Basic Helix-Loop-Helix Transcription Factors: Regulators for Plant Growth Development and Abiotic Stress Responses.International journal of molecular sciences · 2023Review
- Comparative genomics reveals the molecular mechanism of salt adaptation for zoysiagrasses.BMC plant biology · 2022Article
- Regulatory network established by transcription factors transmits drought stress signals in plant.Stress biology · 2022Review
- A Survey of Enhanced Cold Tolerance and Low-Temperature-Induced Anthocyanin Accumulation in a NovelPlants (Basel, Switzerland) · 2022Article
- Comprehensive Profiling of Tubby-Like Proteins in Soybean and Roles of theFrontiers in plant science · 2022Article
- Genome-wide Analysis of Basic Helix-Loop-Helix Family Genes and Expression Analysis in Response to Drought and Salt Stresses inInternational journal of molecular sciences · 2021Article
- Overexpression of ArabidopsisPlant signaling & behavior · 2020Article
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Authors and funding
8 authors at 3 institutions in 2 countries.
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Abstract
key messageZjICE2 works as a positive regulator in abiotic stress responses and ZjICE2 is a valuable genetic resource to improve abiotic stress tolerance in the molecular breeding program of Zoysia japonica. The basic helix-loop-helix (bHLH) family transcription factors (TFs) play an important role in response to biotic or abiotic stresses in plants. However, the functions of bHLH TFs in Zoysia japonica, one of the warm-season turfgrasses, remain poorly understood. Here, we identified ZjICE2 from Z. japonica, a novel MYC-type bHLH transcription factor that was closely related to ICE homologs in the phylogenetic tree, and its expression was regulated by various abiotic stresses. Transient expression of ZjICE2-GFP in onion epidermal cells revealed that ZjICE2 was a nuclear-localized protein. Also, ZjICE2 bound the MYC cis-element in the promoter of dehydration responsive element binding 1 of Z. japonica (ZjDREB1) using yeast one-hybrid assay. A phenotypic analysis showed that overexpression of the ZjICE2 in Arabidopsis enhanced tolerance to cold, drought, and salt stresses. The transgenic Arabidopsis and Z. japonica accumulated more transcripts of cold-responsive DREB/CBFs and their downstream genes than the wild type (WT) after cold treatment. Furthermore, the transgenic plants exhibited an enhanced Reactive oxygen species (ROS) scavenging ability, which resulted in an efficient maintenance of oxidant-antioxidant homeostasis. In addition, overexpression of the ZjICE2 in Z. japonica displayed intensive cold tolerance with increases in chlorophyll contents and photosynthetic efficiency. Our study suggests that ZjICE2 works as a positive regulator in abiotic stress responses and the ICE-DREB/CBFs response pathway involved in cold stress tolerance is also conserved in Z. japonica. These results provide a valuable genetic resource for the molecular breeding program especially for warm-season grasses as well as other leaf crop plants.
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