Evidence map›Paper›PMID 31896126›Full record

ArticlePediatric research2020

Human ucMSCs seeded in a decellularized kidney scaffold attenuate renal fibrosis by reducing epithelial-mesenchymal transition via the TGF-β/Smad signaling pathway.

Dong Hu, Deying Zhang, Bo Liu, Yang Liu, Yu Zhou, Yihang Yu, Lianju Shen, Chunlan Long, Dan Zhang, Xing Liu and 7 more

Open access · bronzeAbstract read
PubMed Publisher
In one paragraph

Article in Pediatric research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed, 1 pooled it
2.2field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 1 synthesis or guideline pooled it, 20 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Review
  4. Review
  5. Article
  6. Review
  7. Article
  8. Umbilical cord mesenchymal stromal cells-from bench to bedside.Frontiers in cell and developmental biology · 2022
    Review
  9. Review
  10. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

17 authors at 7 institutions in 3 countries.

Dong HuDepartment of Urology, Children's Hospital of Chongqing Medical University, 400014, Chongqing, China.
Deying ZhangDepartment of Urology, Children's Hospital of Chongqing Medical University, 400014, Chongqing, China. zdy@hospital.cqmu.edu.cn.
Bo LiuDepartment of Urology, Children's Hospital of Chongqing Medical University, 400014, Chongqing, China.
Yang LiuDepartment of Radiology, The Sixth Affiliated Hospital of Sun Yat-sen University, 510655, Guangzhou, China.
Yu ZhouDepartment of Urology, Children's Hospital of Chongqing Medical University, 400014, Chongqing, China.
Yihang YuDepartment of Urology, Children's Hospital of Chongqing Medical University, 400014, Chongqing, China.
Lianju ShenChongqing Key Laboratory of Children Urogenital Development and Tissue Engineering, Chongqing Key Laboratory of Pediatrics, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders, China International Science and Technology Cooperation base of Child development and Critical Disorders, Children's Hospital of Chongqing Medical University, 400014, Chongqing, China.
Chunlan LongChongqing Key Laboratory of Children Urogenital Development and Tissue Engineering, Chongqing Key Laboratory of Pediatrics, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders, China International Science and Technology Cooperation base of Child development and Critical Disorders, Children's Hospital of Chongqing Medical University, 400014, Chongqing, China.
Dan ZhangChongqing Key Laboratory of Children Urogenital Development and Tissue Engineering, Chongqing Key Laboratory of Pediatrics, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders, China International Science and Technology Cooperation base of Child development and Critical Disorders, Children's Hospital of Chongqing Medical University, 400014, Chongqing, China.
Xing LiuDepartment of Urology, Children's Hospital of Chongqing Medical University, 400014, Chongqing, China.
Tao LinDepartment of Urology, Children's Hospital of Chongqing Medical University, 400014, Chongqing, China.
Dawei HeDepartment of Urology, Children's Hospital of Chongqing Medical University, 400014, Chongqing, China.
Tao XuBio-manufacturing Center, Department of Mechanical Engineering, Tsinghua University, 100084, Beijing, China.
Peter TimashevInstitute for Regenerative Medicine, Sechenov University, 8-2 Trubetskaya Street, 119991, Moscow, Russia.
Denis ButnaruResearch Institute for Uronephrology, Sechenov First Moscow State Medical University, 119991, Moscow, Russia.
Yuanyuan ZhangWake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine, Winston-Salem, NC, 27101, USA.
Guanghui WeiDepartment of Urology, Children's Hospital of Chongqing Medical University, 400014, Chongqing, China. u806806@cqmu.deu.cn.
Children's Hospital of Chongqing Medical University · CNChina International Science and Technology Cooperation · CNChongqing Medical University · CNSechenov University · RUSun Yat-sen University · CNTsinghua University · CNWake Forest University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundRenal fibrosis occurs largely through epithelial-mesenchymal transition (EMT). This study explored the beneficial effects of a human umbilical cord mesenchymal stem cell-loaded decellularized kidney scaffold (ucMSC-DKS) on renal fibrosis in a rodent model of post-transplantation renal failure, and the underlying mechanism.

methodsRat-derived DKSs were examined after preparation, and then recellularized with human ucMSCs to prepare cell-loaded patches. A rat model of renal failure was established after subtotal nephrectomy (STN). The cell patches were transplanted to remnant kidneys. Changes in renal function, histology, EMT, and proteins related to the transforming growth factor-β (TGF-β)/Smad signaling pathway in the remnant kidneys were examined 8 weeks after surgery, compared with non-cell patch controls.

resultsThe DKSs were acellular and porous, with rich cytokine and major extracellular matrix components. The ucMSCs were distributed uniformly in the DKSs. Renal function was improved, renal fibrosis and EMT were reduced, and the TGF-β/Smad signaling pathway was inhibited compared with controls at 8 weeks after ucMSC-DKS patch transplantation.

conclusionsThe ucMSC-DKS restores renal function and reduces fibrosis by reducing EMT via the TGF-β/Smad signaling pathway in rats that have undergone STN. It provides an alternative for renal fibrosis treatment.

Indexed as

Epithelial-Mesenchymal TransitionSignal TransductionAnimalsCells, CulturedCytokinesExtracellular MatrixFibrosisGene Expression ProfilingHumansInflammationKidneyKidney TransplantationMaleMesenchymal Stem CellsMesenchymal Stem Cell TransplantationMicroscopy, Electron, ScanningCytokinesSmad ProteinsTgfb1 protein, ratTransforming Growth Factor beta1

Identifiers

PMID31896126
OpenAlexW2997601709

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.