Evidence map›Paper›PMID 31894536›Full record

ArticleJournal of endocrinological investigation2020

Thyroid hormones T3 and T4 regulate human luteinized granulosa cells, counteracting apoptosis and promoting cell survival.

V Di Paolo, C Mangialardo, C Zacà, M Barberi, E Sereni, A Borini, M Centanni, G Coticchio, C Verga-Falzacappa, R Canipari

Abstract read
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In one paragraph

Article in Journal of endocrinological investigation, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
3.2field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 27 citations in OpenAlex.

  1. The protective role of α-lipoic acid in hypothyroidism-related ovarian damage.Naunyn-Schmiedeberg's archives of pharmacology · 2026
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  8. The Thyroid Hormone Axis and Female Reproduction.International journal of molecular sciences · 2023
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

V Di PaoloDAHFMO, Unit of Histology and Medical Embryology, Sapienza, University of Rome, Via A. Scarpa 14, 00161, Rome, Italy.
C MangialardoDepartment of Medical‑Surgical Sciences and Biotechnologies, Sapienza, University of Rome, Rome, Italy.
C Zacà9.Baby, Family and Fertility Center, Via Dante 15, 40125, Bologna, Italy.
M Barberi9.Baby, Family and Fertility Center, Via Dante 15, 40125, Bologna, Italy.
E Sereni9.Baby, Family and Fertility Center, Via Dante 15, 40125, Bologna, Italy.
A Borini9.Baby, Family and Fertility Center, Via Dante 15, 40125, Bologna, Italy.
M CentanniDepartment of Medical‑Surgical Sciences and Biotechnologies, Sapienza, University of Rome, Rome, Italy.
G Coticchio9.Baby, Family and Fertility Center, Via Dante 15, 40125, Bologna, Italy.
C Verga-FalzacappaDepartment of Medical‑Surgical Sciences and Biotechnologies, Sapienza, University of Rome, Rome, Italy.
R CanipariDAHFMO, Unit of Histology and Medical Embryology, Sapienza, University of Rome, Via A. Scarpa 14, 00161, Rome, Italy. rita.canipari@uniroma1.it.ORCID http://orcid.org/0000-0003-3481-6908
Sapienza University of Rome · ITBambino Gesù Children's Hospital · IT

Funding

Sapienza Università di Roma Finanziamenti Ateneo per la ricerca 2018
6 · The paper itself

Abstract

purposeFine and balanced regulation of cell proliferation and apoptosis are key to achieve ovarian follicle development from the primordial to the preovulatory stage and therefore assure female reproductive function. While gonadotropins are the major and most recognized regulators of follicle cell growth and function, other factors, both systemic and local, play equally important roles. This work is aimed at evaluating the effects of thyroid hormones (THs) on human granulosa luteinized (hGL) viability.

methodsHuman GL cells derived from assisted reproduction treatments were exposed to T3 or T4. Cell viability was evaluated by MTT assay. Apoptosis was evaluated by the TUNEL assay and active caspase-3 staining. StAR, CYP19A1,Caspase-3, P53 and BAX mRNA were evaluated by real-time PCR. LC3-I/-II, AKT and pAKT were evaluated by western blot.

resultsT3 and T4 promoted cell viability in a dose-dependent modality and modulate StAR and CYP19A1 expression. T3 and to a lesser extent T4 mitigated cell death induced by serum starvation by inhibition of caspase-3 activity and expression of P53 and BAX; and attenuate cell death experimentally induced by C2-ceramide. Cell death derived from starvation appeared to be involved in autophagic processes, as the levels of autophagic markers (LC3-II/LC3-I ratio) decreased when starved cells were exposed to T3 and T4. This effect was associated with an increase in pAkt levels.

conclusionFrom the present study, THs emerge as potent anti-apoptotic agents in hGL cells. This effect is achieved by inhibiting the apoptosis signalling pathway of BAX and caspase-3, while maintaining active the PI3K/AKT pathway.

Indexed as

ApoptosisCells, CulturedCell SurvivalDose-Response Relationship, DrugFemaleGranulosa CellsHumansLuteal CellsThyroxineTriiodothyronineThyroxineTriiodothyronineApoptosisGranulosa luteal cellsHuman ovaryThyroid hormones

Identifiers

PMID31894536
OpenAlexW2997803424

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.