Evidence map›Paper›PMID 31894467›Full record

ArticleVirus genes2020

Single amino acid substitutions in the coat protein and RNA-dependent RNA polymerase alleviated the virulence of Cucumber green mottle mosaic virus and conferred cross protection against severe infection.

Jin Liu, Xiang-Dong Li, Shuai Xu

Abstract read
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In one paragraph

Article in Virus genes, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
2.5field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 19 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Jin LiuCollege of Landscape Science and Engineering, Shandong Agriculture and Engineering University, Jinan, 250100, Shandong, China. liujincc612@126.com.ORCID http://orcid.org/0000-0002-0337-1531
Xiang-Dong LiShandong Provincial Key Laboratory for Agricultural University, Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Tai'an, 271018, Shandong, China.
Shuai XuShandong Provincial Key Laboratory for Agricultural University, Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Tai'an, 271018, Shandong, China.
Shandong Agricultural University · CNShandong University · CN

Funding

National Natural Science Foundation of China 31571984National Natural Science Foundation of China 31720103912
6 · The paper itself

Abstract

Cross protection is a promising alternate to control Cucumber green mottle mosaic virus (CGMMV) which is of increasing economic importance to cucurbit production worldwide. One major factor confronting the application of cross protection to control CGMMV is the scarcity of available mild mutants. The objective of this paper was to screen attenuated mutants of CGMMV and evaluate their potential in cross protection. An infectious cDNA clone of CGMMV, pCGMMV, was obtained by cloning intron-containing CGMMV genome to modified pCambia0390 vector with the Cauliflower mosaic virus 35S promoter. Five pCGMMV-derived mutants were obtained via site-directed mutagenesis and inoculated to Nicotiana benthamiana plants for symptom observation. The attenuated CGMMV mutants were evaluated for their efficiency in cross protection. The intron-containing clone pCGMMV induced similar disease symptoms and accumulated similar titres of virus in N. benthamiana plants as wild-type CGMMV. Mutations of aspartic acid at position 89 in the coat protein to alanine (D

Indexed as

Amino Acid SubstitutionCapsid ProteinsCucumis sativusGenome, ViralMutagenesis, Site-DirectedNicotianaPlant DiseasesRNA-Dependent RNA PolymeraseTobamovirusVirulenceCapsid ProteinsRNA-Dependent RNA PolymeraseCGMMVCoat proteinCross protectionMild mutantsRNA-dependent RNA polymerase

Identifiers

PMID31894467
OpenAlexW2998308752

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.