Evidence map›Paper›PMID 31892728›Full record

ArticleScientific reports2019

An inhibitor of apoptosis protein (EsIAP1) from Chinese mitten crab Eriocheir sinensis regulates apoptosis through inhibiting the activity of EsCaspase-3/7-1.

Chen Qu, Jiejie Sun, Qingsong Xu, Xiaojing Lv, Wen Yang, Feifei Wang, Ying Wang, Qilin Yi, Zhihao Jia, Lingling Wang and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed, 1 pooled it
1.0field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 1 synthesis or guideline pooled it, 27 citations in OpenAlex.

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  10. A survey of labour epidural practices at obstetric anesthesia fellowship programs in the United States.Canadian journal of anaesthesia = Journal canadien d'anesthesie · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Chen QuLiaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, 116023, China.
Jiejie SunLiaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, 116023, China.
Qingsong XuLiaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, 116023, China.
Xiaojing LvLiaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, 116023, China.
Wen YangLiaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, 116023, China.
Feifei WangLiaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, 116023, China.
Ying WangLiaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, 116023, China.
Qilin YiLiaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, 116023, China.
Zhihao JiaLaboratory of Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266235, China.
Lingling WangLiaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, 116023, China.
Linsheng SongLiaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, 116023, China. lshsong@dlou.edu.cn.
Dalian Ocean University · CNQingdao National Laboratory for Marine Science and Technology · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Inhibitor of apoptosis proteins (IAPs) maintain the balance between cell proliferation and cell death by inhibiting caspase activities and mediating immune responses. In the present study, a homolog of IAP (designated as EsIAP1) was identified from Chinese mitten crab Eriocheir sinensis. EsIAP1 consisted of 451 amino acids containing two baculoviral IAP repeat (BIR) domains with the conserved Cx2 Cx6 Wx3 Dx5 Hx6 C motifs. EsIAP1 mRNA was expressed in various tissues and its expression level in hemocytes increased significantly (p < 0.01) at 12-48 h after lipopolysaccharide stimulation. In the hemocytes, EsIAP1 protein was mainly distributed in the cytoplasm. The hydrolytic activity of recombinant EsCaspase-3/7-1 against the substrate Ac-DEVD-pNA decreased after incubation with rEsIAP1. Moreover, rEsIAP1 could directly combine with rEsCaspase-3/7-1 in vitro. After EsIAP1 was interfered by dsRNA, the mRNA expression and the hydrolytic activity of EsCaspase-3/7-1 increased significantly, which was 2.26-fold (p < 0.05) and 1.71-fold (p < 0.05) compared to that in the dsGFP group, respectively. These results collectively demonstrated that EsIAP1 might play an important role in apoptosis pathway by regulating the activity of EsCaspase-3/7-1 in E. sinensis.

Indexed as

AnimalsApoptosisArthropod ProteinsBaculoviral IAP Repeat-Containing 3 ProteinBrachyuraCaspase 3Caspase 7Gene Expression RegulationArthropod ProteinsBaculoviral IAP Repeat-Containing 3 ProteinCaspase 3Caspase 7

Identifiers

PMID31892728
PMCPMC6938513
OpenAlexW2997808605

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.