Evidence map›Paper›PMID 31892315›Full record

ArticleMolecular medicine (Cambridge, Mass.)2019

HMGB1 concentration measurements in trauma patients: assessment of pre-analytical conditions and sample material.

William Ottestad, Ingrid N Rognes, Erlend Skaga, Cassandra Frisvoll, Guttorm Haraldsen, Torsten Eken, Peter Lundbäck

Open access · goldAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular medicine (Cambridge, Mass.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.1field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 14 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

William OttestadDepartment of Anaesthesiology, Oslo University Hospital, PO Box 4956 Nydalen, NO-0424, Oslo, Norway. williamottestad@gmail.com.ORCID 0000-0002-2435-9363
Ingrid N RognesInstitute of Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway.
Erlend SkagaInstitute of Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway.
Cassandra FrisvollFaculty of Medicine, University of Oslo, Oslo, Norway.
Guttorm HaraldsenK.G. Jebsen Inflammation Research Centre, Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway.
Torsten EkenDepartment of Anaesthesiology, Oslo University Hospital, PO Box 4956 Nydalen, NO-0424, Oslo, Norway.
Peter LundbäckK.G. Jebsen Inflammation Research Centre, Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway.
Oslo University Hospital · NOUniversity of Oslo · NO

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundHMGB1 is a mediator of systemic inflammation in sepsis and trauma, and a promising biomarker in many diseases. There is currently no standard operating procedure for pre-analytical handling of HMGB1 samples, despite that pre-analytical conditions account for a substantial part of the overall error rate in laboratory testing. We hypothesized that the considerable variations in reported HMGB1 concentrations and kinetics in trauma patients could be partly explained by differences in pre-analytical conditions and choice of sample material.

methodsTrauma patients (n = 21) admitted to a Norwegian Level I trauma center were prospectively included. Blood was drawn in K

resultsArterial HMGB1 concentrations were consistently lower than venous concentrations in simultaneously obtained samples (arterial = 0.60 x venous; 95% CI 0.30-0.90). Concentrations in plasma and serum showed a strong linear correlation, however wide limits of agreement. Storage of blood samples at room temperature prior to centrifugation resulted in an exponential increase in plasma concentrations after ≈6 h. HMGB1 concentrations were fairly stable in centrifuged plasma samples subjected to long-term storage and freeze/thaw cycles. We were not able to detect HMGB1 in either serum or plasma from our trauma patients using western blotting.

conclusionsArterial and venous HMGB1 concentrations cannot be directly compared, and concentration values in plasma and serum must be compared with caution due to wide limits of agreement. Although HMGB1 levels in clinical samples from trauma patients are fairly stable, strict adherence to a pre-analytical protocol is advisable in order to protect sample integrity. Surprisingly, we were unable to detect HMGB1 utilizing standard western blot analysis.

Indexed as

AdolescentAdultAgedArteriesBiomarkersBlood Specimen CollectionFemaleFreezingHMGB1 ProteinHumansMaleMiddle AgedProspective StudiesTemperatureTime FactorsVeinsBiomarkersHMGB1 ProteinBlotting, westernEnzyme-linked immunosorbent assayHMGB1 proteinPre-analytical phaseWounds and injuries

Identifiers

PMID31892315
PMCPMC6938620
OpenAlexW2997950529

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