ArticleNuclear medicine and biology
High-throughput radio-TLC analysis.
Article in Nuclear medicine and biology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 34 citations in OpenAlex.
- Droplet-Based Radiosynthesis and High-Throughput Optimization of Vinyl Sulfone Prosthetic Group ([Molecules (Basel, Switzerland) · 2026Article
- Co-57-based intercomparison of TLC scanners and dose calibrators for low-activity radiochemical purity assessment in radiopharmaceutical quality control.EJNMMI radiopharmacy and chemistry · 2026Article
- Chemical Tools to Characterize the Coordination Chemistry of Radionuclides for Radiopharmaceutical Applications.Chemical reviews · 2025Review
- Development of a radio thin-layer chromatography scanner: Design and qualification.EJNMMI radiopharmacy and chemistry · 2025Article
- Rapid Concentration of Ga-68 and Proof-of-Concept Microscale Labeling of [Molecules (Basel, Switzerland) · 2024Article
- Revolutionizing cancer treatment: The role of radiopharmaceuticals in modern cancer therapy.Precision radiation oncology · 2024Review
- Development of High-Throughput Experimentation Approaches for Rapid Radiochemical Exploration.Journal of the American Chemical Society · 2024Article
- Scalable droplet-based radiosynthesis of [Lab on a chip · 2024Article
- Proof-of-concept optimization of a copper-mediatedLab on a chip · 2023Article
- Accelerating radiochemistry development: Automated robotic platform for performing up to 64 droplet radiochemical reactions in a morning.Chemical engineering journal (Lausanne, Switzerland : 1996) · 2023Article
- Detrimental impact of aqueous mobile phases on 18F-labelled radiopharmaceutical analysisAnalytical methods : advancing methods and applications · 2023Article
- Standardized Uptake Values on SPECT/CT: A Promising Alternative Tool for Treatment Evaluation and Prognosis of Metastatic Neuroendocrine Tumours.Diagnostics (Basel, Switzerland) · 2023Article
- A rapid and systematic approach for the optimization of radio thin-layer chromatography resolution.Journal of chromatography. A · 2023Article
- Rapid Purification and Formulation of Radiopharmaceuticals via Thin-Layer Chromatography.Molecules (Basel, Switzerland) · 2022Article
- Radiolabeling and quality control of therapeutic radiopharmaceuticals: optimization, clinical implementation and comparison of radio-TLC/HPLC analysis, demonstrated by [EJNMMI radiopharmacy and chemistry · 2022Article
- Microliter-scale reaction arrays for economical high-throughput experimentation in radiochemistry.Scientific reports · 2022Article
- The New Radiolabeled PeptideCancers · 2022Article
- Economical Production of Radiopharmaceuticals for Preclinical Imaging Using Microdroplet Radiochemistry.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Optimization of Radiochemical Reactions using Droplet Arrays.Journal of visualized experiments : JoVE · 2021Article
- Cerenkov Luminescence Imaging in the Development and Production of Radiopharmaceuticals.Frontiers in physics · 2021Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
introductionRadio thin layer chromatography (radio-TLC) is commonly used to analyze purity of radiopharmaceuticals or to determine the reaction conversion when optimizing radiosynthesis processes. In applications where there are few radioactive species, radio-TLC is preferred over radio-high-performance liquid chromatography due to its simplicity and relatively quick analysis time. However, with current radio-TLC methods, it remains cumbersome to analyze a large number of samples during reaction optimization. In a couple of studies, Cerenkov luminescence imaging (CLI) has been used for reading radio-TLC plates spotted with a variety of isotopes. We show that this approach can be extended to develop a high-throughput approach for radio-TLC analysis of many samples.
methodsThe high-throughput radio-TLC analysis was carried out by performing parallel development of multiple radioactive samples spotted on a single TLC plate, followed by simultaneous readout of the separated samples using Cerenkov imaging. Using custom-written MATLAB software, images were processed and regions of interest (ROIs) were drawn to enclose the radioactive regions/spots. For each sample, the proportion of integrated signal in each ROI was computed. Various crude samples of [
resultsBenefiting from a parallel developing process and high resolution of CLI-based readout, total analysis time for eight [
conclusionsCerenkov imaging in combination with parallel developing of multiple samples on a single TLC plate proved to be a practical method for rapid, high-throughput radio-TLC analysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.