ArticleRegenerative therapy2019
Understanding the formation and behaviors of droplets toward consideration of changeover during cell manufacturing.
Article in Regenerative therapy, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 13 citations in OpenAlex.
- Metagenomic-driven predictive biosafety and contamination traceability in stem cell manufacturing: Translating outbreak genomics into regenerative medicine.Regenerative therapy · 2026Review
- Cleaning methods for biosafety cabinet to eliminate residual mycoplasmas, viruses, and endotoxins after changeover.Regenerative therapy · 2025Article
- Approach of design for air mass balance in an aseptic processing area for cell-based products.Regenerative therapy · 2025Article
- Changeover method for biosafety cabinets using ozone gas.PloS one · 2025Article
- Decreasing electricity costs of clean room for cell products during non-operation.Regenerative therapy · 2024Article
- Evaluation of cleaning methods for change-over after the processing of cell products to avoid cross-contamination risk.Regenerative therapy · 2024Article
- Operator-derived particles and falling bacteria in biosafety cabinets.Regenerative therapy · 2024Article
- Volatile organic compounds and ionic substances contamination in cell processing facilities during rest period; a preliminary assessment of exposure to cell processing operators.Regenerative therapy · 2023Article
- Effect of disinfectants and manual wiping for processing the cell product changeover in a biosafety cabinet.Regenerative therapy · 2023Article
- Cross-contamination risk and decontamination during changeover after cell-product processing.Regenerative therapy · 2023Article
- Stress and motivation of cell processing operators: A pilot study of an online questionnaire survey.Regenerative therapy · 2022Article
- Approach of resource expenditure estimation toward mechanization in the manufacturing of cell-based products.Regenerative therapy · 2022Article
- Speech Sounds and the Production of Droplets and Aerosols.Internal medicine (Tokyo, Japan) · 2021Article
- The environmental risk assessment of cell-processing facilities for cell therapy in a Japanese academic institution.PloS one · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Preventing the contamination of processed cells is required for achieving reproducible manufacturing. A droplet is one of the potential causes contamination in cell manufacturing. The present study elucidates the formation mechanism and characteristics of droplets based on the observation and detection of droplets on the base surface of the biological safety cabinet (BSC) where cell processing is conducted under unidirectional airflow. Pouring fluorescent solution into the vessel using a measuring pipette was conducted to visualize the formation of droplets by videos as well as visual detection by blacklight irradiation on the base surface of the BSC. The experiments revealed that airborne and non-airborne droplets emerged from bursting bubbles, which formed when the entire solution was pushed out of the measuring pipette. Therefore, the improving procedure of pouring technique when entire solution was not pushed out of the pipette realized no formation of the droplets due to the prevention of emergence of bubble. In addition, an alternative procedure in which the entire solution was poured into the deep point of the test tube prevented the flying of non-airborne droplets outside the tube, while airborne droplets that escaped the tube rode the airflow of BSC. These results suggested a method for the prevention of the droplet formation, as well as the deposit control of droplets onto the surface in BSC, leading to cleanup area in the BSC for changeover with environment continuity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.