Evidence map›Paper›PMID 31890588›Full record

ArticleMetabolic engineering communications2020

Development of both type I-B and type II CRISPR/Cas genome editing systems in the cellulolytic bacterium

Julie E Walker, Anthony A Lanahan, Tianyong Zheng, Camilo Toruno, Lee R Lynd, Jeffrey C Cameron, Daniel G Olson, Carrie A Eckert

Open access · goldAbstract read
In one paragraph

Article in Metabolic engineering communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.

0numbers the graph read from it
0cells of the map it votes in
43citing papers in PubMed
4.0field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

43 citing papers in PubMed, 103 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
  5. A diverse single-stranded DNA-annealing protein library enables efficient genome editing across bacterial phyla.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  6. Article
  7. Article
  8. Current models in bacterial hemicellulase-encoding gene regulation.Applied microbiology and biotechnology · 2024
    Review
  9. Review
  10. Article
  11. Review
  12. Review
  13. Application of functional genomics for domestication of novel non-model microbes.Journal of industrial microbiology & biotechnology · 2024
    Review
  14. The potential of native and engineered Clostridia for biomass biorefining.Frontiers in bioengineering and biotechnology · 2024
    Review
  15. Article
  16. Article
  17. Ethanol tolerance in engineered strains of Clostridium thermocellum.Biotechnology for biofuels and bioproducts · 2023
    Article
  18. Article
  19. Review
  20. Endogenous CRISPR/Cas systems for genome engineering in the acetogensFrontiers in bioengineering and biotechnology · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 1 country.

Julie E WalkerRenewable and Sustainable Energy Institute, University of Colorado, Boulder, CO, 80303, USA.
Anthony A LanahanCenter for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, 37830, USA.
Tianyong ZhengCenter for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, 37830, USA.
Camilo TorunoCenter for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, 37830, USA.
Lee R LyndCenter for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, 37830, USA.
Jeffrey C CameronRenewable and Sustainable Energy Institute, University of Colorado, Boulder, CO, 80303, USA.
Daniel G OlsonCenter for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, 37830, USA.
Carrie A EckertRenewable and Sustainable Energy Institute, University of Colorado, Boulder, CO, 80303, USA.
Dartmouth College · USOak Ridge National Laboratory · USNational Renewable Energy Laboratory · USUniversity of Colorado Boulder · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The robust lignocellulose-solubilizing activity of

Indexed as

5-FOA, 5-fluoroorotic acidCas9Cas9n, nickase Cas9CFU, colony forming unitClostridium thermocellumCRISPRCRISPR/Cas, Clustered Regularly-Interspaced Short Palindromic Repeat/CRISPR associatedHDR, homology-directed repairHR, homologous recombinationPAM, protospacer adjacent motifRNP, Cas9-sgRNA ribonucleoproteinsgRNA, single guide RNAThermophilic recombineeringTm, thiamphenicolType I–B

Identifiers

PMID31890588
PMCPMC6926293
OpenAlexW2989701788

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.