Evidence map›Paper›PMID 31890272›Full record

ArticleZoological letters2019

A self-marker-like protein governs hemocyte allorecognition in

Masaki Ema, Taizo Okada, Miki Takahashi, Masato Uchiyama, Hideo Kubo, Hideaki Moriyama, Hitoshi Miyakawa, Midori Matsumoto

Open access · goldAbstract read
In one paragraph

Article in Zoological letters, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 2 countries.

Masaki Ema *1Department of Biological Sciences and Informatics, Keio University, 3-14-1, Hiyoshi, Kouhoku-ku, Yokohama, 223-8522 Japan.
Taizo Okada *1Department of Biological Sciences and Informatics, Keio University, 3-14-1, Hiyoshi, Kouhoku-ku, Yokohama, 223-8522 Japan.
Miki Takahashi *1Department of Biological Sciences and Informatics, Keio University, 3-14-1, Hiyoshi, Kouhoku-ku, Yokohama, 223-8522 Japan.
Masato Uchiyama *1Department of Biological Sciences and Informatics, Keio University, 3-14-1, Hiyoshi, Kouhoku-ku, Yokohama, 223-8522 Japan.
Hideo Kubo2Tokyo Metropolitan Institute of Medical Science, 2-1-6, Kami-Kitazawa, Setagaya-ku, Tokyo, 156-8506 Japan.
Hideaki Moriyama3School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE USA.
Hitoshi Miyakawa4Laboratory of Environmental Physiology, Center for Bioscience Research and Education, Utsunomiya University, 350 Mine-machi, Utsunomiya, Tochigi, 321-8505 Japan.
Midori Matsumoto1Department of Biological Sciences and Informatics, Keio University, 3-14-1, Hiyoshi, Kouhoku-ku, Yokohama, 223-8522 Japan.ORCID 0000-0003-4838-6179
Keio University · JPTokyo Metropolitan Institute of Medical Science · JPUniversity of Nebraska–Lincoln · USUtsunomiya University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundSelf-incompatibility, fusion/non-fusion reactions, and contact reactions (CRs) have all been identified as allorecognition phenomena in ascidians. CR is a reaction characteristic of the hemocytes of

resultsWe initially generated a CR-inducing monoclonal antibody against the complete hemocyte membrane-protein complement (mAb11B16B10). This antibody was identified based on the differential induction of PO activity in individual organisms. The level of PO activity induced by this antibody in individual ascidians was consistent with the observed CR-induced PO activity. mAb11B16B10 recognized a series of 12 spots corresponding to a 100-kDa protein, with differing isoelectric points (pIs). A comparison of the 2D electrophoresis gels of samples from CR-reactive/non-reactive individuals revealed that some spots in this series in hemocytes were common to the CR-non-inducible individuals, but not to CR-inducible individuals. We cloned the corresponding gene and named it

conclusionWe generated a CR-inducing monoclonal antibody (mAb11B16B10) that recognized a series of novel membrane proteins (HrSMLP1) in the hemocytes of

Indexed as

AllorecognitionAscidianContact reactionHalocynthia roretziHemocyte

Identifiers

PMID31890272
PMCPMC6916515
OpenAlexW2995877773

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.