Evidence map›Paper›PMID 31889981›Full record

ArticlePlant methods2019

Virus-induced gene silencing (VIGS) in

Julia Schachtsiek, Tajammul Hussain, Khadija Azzouhri, Oliver Kayser, Felix Stehle

Open access · goldAbstract read
In one paragraph

Article in Plant methods, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
5.2field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 46 citations in OpenAlex.

  1. From Model Plants toPlants (Basel, Switzerland) · 2026
    Review
  2. Genetic Advances inPlants (Basel, Switzerland) · 2026
    Review
  3. Article
  4. Understanding the role of oxylipins inFrontiers in plant science · 2025
    Review
  5. Article
  6. Virus-Induced Gene Silencing in the Tea Plant (Plants (Basel, Switzerland) · 2023
    Article
  7. Horticulture research · 2023
    Article
  8. Review
  9. Article
  10. Review
  11. Comparative genomics of flowering behavior inFrontiers in plant science · 2023
    Article
  12. Article
  13. UnderstandingPlants (Basel, Switzerland) · 2022
    Review
  14. Article
  15. Frontiers in plant science · 2022
    Article
  16. Review
  17. Review
  18. Manipulation of Cannabinoid BiosynthesisFrontiers in plant science · 2021
    Article
  19. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Julia SchachtsiekLaboratory of Technical Biochemistry, Department of Biochemical and Chemical Engineering, TU Dortmund University, Dortmund, Germany.
Tajammul HussainLaboratory of Technical Biochemistry, Department of Biochemical and Chemical Engineering, TU Dortmund University, Dortmund, Germany.
Khadija AzzouhriLaboratory of Technical Biochemistry, Department of Biochemical and Chemical Engineering, TU Dortmund University, Dortmund, Germany.
Oliver KayserLaboratory of Technical Biochemistry, Department of Biochemical and Chemical Engineering, TU Dortmund University, Dortmund, Germany.
Felix StehleLaboratory of Technical Biochemistry, Department of Biochemical and Chemical Engineering, TU Dortmund University, Dortmund, Germany.ORCID 0000-0002-3134-6253
TU Dortmund University · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe raised demand of cannabis as a medicinal plant in recent years led to an increased interest in understanding the biosynthetic routes of cannabis metabolites. Since there is no established protocol to generate stable gene knockouts in cannabis, the use of a virus-induced gene silencing (VIGS) method, resulting in a gene knockdown, to study gene functions is desirable.

resultsFor this, a computational approach was employed to analyze the

conclusionsThe applied VIGS-method can be used for reverse genetic studies in

Indexed as

Cannabis sativaCotton leaf crumple virus (CLCrV)Post-transcriptional gene silencing (PTGS)Virus-induced gene silencing

Identifiers

PMID31889981
PMCPMC6931244
OpenAlexW2998255740

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.