ArticlePlant methods2019
Fluctuating light experiments and semi-automated plant phenotyping enabled by self-built growth racks and simple upgrades to the IMAGING-PAM.
Article in Plant methods, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Projecting 2D top-view of PSII efficiency onto 3D plant models to quantify PSII efficiency across canopy layers.Plant methods · 2026Article
- Bestrophin-like protein 4 is involved in photosynthetic acclimation to light fluctuations in Chlamydomonas.Plant physiology · 2024Article
- Cyclic electron flow compensates loss of PGDH3 and concomitant stromal NADH reduction.Scientific reports · 2024Article
- An open-source controller to build a dynamic light intensity setup.Plant methods · 2024Article
- Probing the physiological role of the plastid outer-envelope membrane using the oemiR plasmid collection.G3 (Bethesda, Md.) · 2023Article
- Pyruvate, phosphate dikinase regulatory protein impacts light response of C4 photosynthesis in Setaria viridis.Plant physiology · 2022Article
- The Arabidopsis T-DNA mutant SALK_008491 carries a 14-kb deletion on chromosome 3 that provides rare insights into the plant response to dynamic light stress.Plant direct · 2022Article
- Photoperiod Stress inFrontiers in plant science · 2022Article
- Multiparameter in vivo imaging in plants using genetically encoded fluorescent indicator multiplexing.Plant physiology · 2021Review
- Impact of ion fluxes across thylakoid membranes on photosynthetic electron transport and photoprotection.Nature plants · 2021Article
- Stromal NADH supplied by PHOSPHOGLYCERATE DEHYDROGENASE3 is crucial for photosynthetic performance.Plant physiology · 2021Article
- The topology of plastid inner envelope potassium cation efflux antiporter KEA1 provides new insights into its regulatory features.Photosynthesis research · 2020Article
- Comparative analysis of thylakoid protein complexes in state transition mutants nsi and stn7: focus on PSI and LHCII.Photosynthesis research · 2020Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundOver the last years, several plant science labs have started to employ fluctuating growth light conditions to simulate natural light regimes more closely. Many plant mutants reveal quantifiable effects under fluctuating light despite being indistinguishable from wild-type plants under standard constant light. Moreover, many subtle plant phenotypes become intensified and thus can be studied in more detail. This observation has caused a paradigm shift within the photosynthesis research community and an increasing number of scientists are interested in using fluctuating light growth conditions. However, high installation costs for commercial controllable LED setups as well as costly phenotyping equipment can make it hard for small academic groups to compete in this emerging field.
resultsWe show a simple do-it-yourself approach to enable fluctuating light growth experiments. Our results using previously published fluctuating light sensitive mutants,
conclusionsWith material costs well below USD$1000, it is possible to setup a fluctuating light rack including a constant light control shelf for comparison. This allows more scientists to perform experiments closer to natural light conditions and contribute to an emerging research field. A small addition to the IMAGING-PAM hardware not only increases sample throughput but also enables larger-scale plant phenotyping with automated data analysis.
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