ArticleScientific reports2019
Characterization and functional roles of paternal RNAs in 2-4 cell bovine embryos.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
- Heat Stress and the Bull Sperm Transcriptome: Molecular Mechanisms, Fertility, and Epigenetic Inheritance.Animals : an open access journal from MDPI · 2026Review
- Epididymal extracellular vesicles harbor and convey mRNA to sperm for transfer to zygotes.Nucleic acids research · 2026Article
- Systematic mRNA interactome analysis reconceptualizes translational quiescence in bovine sperm.Communications biology · 2025Article
- Decoding bull fertility in vitro: a proteomics exploration from sperm to blastocyst.Reproduction (Cambridge, England) · 2025Article
- Paternal Effects in Mammalian Reproduction: Functional, Environmental, and Clinical Relevance of Sperm Components in Early Embryos and Beyond.Molecular reproduction and development · 2025Review
- Silent cells? Potential for context-dependent gene expression in mature sperm.Proceedings. Biological sciences · 2025Review
- Advanced Skeletal Ossification Is Associated with Genetic Variants in Chronologically Young Beef Heifers.Genes · 2023Article
- Pre-Implantation Bovine Embryo Evaluation-From Optics to Omics and Beyond.Animals : an open access journal from MDPI · 2023Review
- The Sperm Small RNA Transcriptome: Implications beyond Reproductive Disorder.International journal of molecular sciences · 2022Review
- In Silico Identification of lncRNAs Regulating Sperm Motility in the Turkey (International journal of molecular sciences · 2022Article
- Identification of differentially expressed mRNAs and miRNAs in spermatozoa of bulls of varying fertility.Frontiers in veterinary science · 2022Article
- Influence of sire fertility status on conceptus-induced transcriptomic response of the bovine endometrium.Frontiers in cell and developmental biology · 2022Article
- Programming of Embryonic Development.International journal of molecular sciences · 2021Review
- Article
- Gene Expression in Embryos From Norwegian Red Bulls With High or Low Non Return Rate: An RNA-Seq Study of in vivo-Produced Single Embryos.Frontiers in genetics · 2021Article
- The Epigenetics of Gametes and Early Embryos and Potential Long-Range Consequences in Livestock Species-Filling in the Picture With Epigenomic Analyses.Frontiers in genetics · 2021Review
- Post-ejaculation thermal stress causes changes to the RNA profile of sperm in an external fertilizer.Proceedings. Biological sciences · 2020Article
- Mid-Gestation lethality ofInternational journal of molecular sciences · 2020Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Embryos utilize oocyte-donated RNAs until they become capable of producing RNAs through embryonic genome activation (EGA). The sperm's influence over pre-EGA RNA content of embryos remains unknown. Recent studies have revealed that sperm donate non-genomic components upon fertilization. Thus, sperm may also contribute to RNA presence in pre-EGA embryos. The first objective of this study was to investigate whether male fertility status is associated with the RNAs present in the bovine embryo prior to EGA. A total of 65 RNAs were found to be differentially expressed between 2-4 cell bovine embryos derived from high and low fertility sires. Expression patterns were confirmed for protein phosphatase 1 regulatory subunit 36 (PPP1R36) and ataxin 2 like (ATXN2L) in three new biological replicates. The knockdown of ATXN2L led to a 22.9% increase in blastocyst development. The second objective of this study was to characterize the parental origin of RNAs present in pre-EGA embryos. Results revealed 472 sperm-derived RNAs, 2575 oocyte-derived RNAs, 2675 RNAs derived from both sperm and oocytes, and 663 embryo-exclusive RNAs. This study uncovers an association of male fertility with developmentally impactful RNAs in 2-4 cell embryos. This study also provides an initial characterization of paternally-contributed RNAs to pre-EGA embryos. Furthermore, a subset of 2-4 cell embryo-specific RNAs was identified.
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