ArticleBMC microbiology2019
An in vitro collagen perfusion wound biofilm model; with applications for antimicrobial studies and microbial metabolomics.
Article in BMC microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 35 citations in OpenAlex.
- Engineering in vitro models to replicate the complexity of chronic wounds.Nature communications · 2026Review
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- Inhibitory effects of Quercetagetin on the growth, biofilm formation, and virulence factors of bovine mastitis-associatedFrontiers in microbiology · 2026Article
- Nitric Oxide-Releasing Polydimethylsiloxane Sponges with Tunable Porosity.ACS applied materials & interfaces · 2025Article
- Newly developed antibiotics against multidrug-resistant and carbapenem-resistant Gram-negative bacteria: action and resistance mechanisms.Archives of microbiology · 2025Review
- A New Method for Obtaining Monospecies and Binary Cultures of Staphylococcus spp. in Alginate Gel and the Study of the Action of Active Compounds on These Cultures on the Example of Catecholamines.Bulletin of experimental biology and medicine · 2024Article
- Advancements and Challenges of Nanostructured Lipid Carriers for Wound Healing Applications.International journal of nanomedicine · 2024Review
- Anti-Biofilm Efficacy of Commonly Used Wound Care Products in In Vitro Settings.Antibiotics (Basel, Switzerland) · 2023Article
- Therapeutic Potential of an Azithromycin-Colistin Combination against XDRAntibiotics (Basel, Switzerland) · 2023Article
- A Synthetic Polymicrobial Community Biofilm Model Demonstrates Spatial Partitioning, Tolerance to Antimicrobial Treatment, Reduced Metabolism, and Small Colony Variants Typical of Chronic Wound Biofilms.Pathogens (Basel, Switzerland) · 2023Article
- Development of an implantable three-dimensional model of a functional pathogenic multispecies biofilm to study infected wounds.Scientific reports · 2022Article
- TA-AgNPs/Alginate Hydrogel and Its Potential Application as a Promising Antibiofilm Material against Polymicrobial Wound Biofilms Using a Unique Biofilm Flow Model.Microorganisms · 2022Article
- Multi-Strain and -Species Investigation of Volatile Metabolites Emitted from Planktonic and BiofilmMetabolites · 2022Article
- Real-time detection of volatile metabolites enabling species-level discrimination of bacterial biofilms associated with wound infection.Journal of applied microbiology · 2022Article
- Advances in experimental and computational methodologies for the study of microbial-surface interactions at different omics levels.Frontiers in microbiology · 2022Review
- Milieu matters: AnBiofilm · 2021Article
- Microbial fuel cells and their electrified biofilms.Biofilm · 2021Article
- A novel chronic wound biofilm model sustaining coexistence of Pseudomonas aeruginosa and Staphylococcus aureus suitable for testing of antibiofilm effect of antimicrobial solutions and wound dressings.Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society · 2021Article
- Effect of collagen and EPS components on the viscoelasticity ofSoft matter · 2021Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe majority of in vitro studies of medically relevant biofilms involve the development of biofilm on an inanimate solid surface. However, infection in vivo consists of biofilm growth on, or suspended within, the semi-solid matrix of the tissue, whereby current models do not effectively simulate the nature of the in vivo environment. This paper describes development of an in vitro method for culturing wound associated microorganisms in a system that combines a semi-solid collagen gel matrix with continuous flow of simulated wound fluid. This enables culture of wound associated reproducible steady state biofilms under conditions that more closely simulate the dynamic wound environment. To demonstrate the use of this model the antimicrobial kinetics of ceftazidime, against both mature and developing Pseudomonas aeruginosa biofilms, was assessed. In addition, we have shown the potential application of this model system for investigating microbial metabolomics by employing selected ion flow tube mass spectrometry (SIFT-MS) to monitor ammonia and hydrogen cyanide production by Pseudomonas aeruginosa biofilms in real-time.
resultsThe collagen wound biofilm model facilitates growth of steady-state reproducible Pseudomonas aeruginosa biofilms under wound like conditions. A maximum biofilm density of 10
conclusionsThe collagen wound biofilm model has been developed to facilitate growth of reproducible biofilms under wound-like conditions. We have successfully used this method to: (1) evaluate antimicrobial efficacy and kinetics, clearly demonstrating the development of antimicrobial tolerance in biofilm cultures; (2) characterise volatile metabolite production by P. aeruginosa biofilms, demonstrating the potential use of this method in metabolomics studies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.