ArticleEMBO reports2020
Monomeric cohesin state revealed by live-cell single-molecule spectroscopy.
Article in EMBO reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 24 citations in OpenAlex.
- Symmetric control of sister chromatid cohesion establishment.Nucleic acids research · 2023Article
- Multifunctional Oxygenated Particles for Targeted Cancer Drug Delivery and Evaluation with Darkfield Hyperspectral Imaging.ACS omega · 2022Article
- Fold-change of chromatin condensation in yeast is a conserved property.Scientific reports · 2022Article
- Article
- G1-Cyclin2 (Cln2) promotes chromosome hypercondensation in eco1/ctf7 rad61 null cells during hyperthermic stress in Saccharomyces cerevisiae.G3 (Bethesda, Md.) · 2022Article
- Article
- It's all in the numbers: Cohesin stoichiometry.Frontiers in molecular biosciences · 2022Review
- Chromosome loading of cohesin depends on conserved residues in Scc3.Current genetics · 2021Article
- Epigenetic alterations associated with dexamethasone sodium phosphate through DNMT and TET in RPE cells.Molecular vision · 2021Article
- IRX3/5 regulate mitotic chromatid segregation and limb bud shape.Development (Cambridge, England) · 2020Article
- PCNA promotes context-specific sister chromatid cohesion establishment separate from that of chromatin condensation.Cell cycle (Georgetown, Tex.) · 2020Article
- Distinct and overlapping roles of STAG1 and STAG2 in cohesin localization and gene expression in embryonic stem cells.Epigenetics & chromatin · 2020Article
- Monomeric cohesin state revealed by live-cell single-molecule spectroscopy.EMBO reports · 2020Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
The cohesin complex plays an important role in the maintenance of genome stability. Cohesin is composed of four core subunits and a set of regulatory subunits that interact with the core subunits. Less is known about cohesin dynamics in live cells and on the contribution of individual subunits to the overall complex. Understanding the tethering mechanism of cohesin is still a challenge, especially because the proposed mechanisms are still not conclusive. Models proposed to describe tethering depend on either the monomeric cohesin ring or a cohesin dimer. Here, we investigate the role of cohesin dynamics and stoichiometry in live yeast cells at single-molecule resolution. We explore the effect of regulatory subunit deletion on cohesin mobility and found that depletion of different regulatory subunits has opposing effects. Finally, we show that cohesin exists mostly as a canonical monomer throughout the cell cycle, and its monomeric form is independent of its regulatory factors. Our results demonstrate that single-molecule tools have the potential to provide new insights into the cohesin mechanism of action in live cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.