ArticleMolecular systems biology2019
Digitalizing heterologous gene expression in Gram-negative bacteria with a portable ON/OFF module.
Article in Molecular systems biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 58 citations in OpenAlex.
- Accelerated Adaptive Evolution of Gram-Negative Bacteria With a Broad-Host-Range Dual Base Editor.Microbial biotechnology · 2026Article
- Precision Proteolysis of Triosephosphate Isomerase ofACS synthetic biology · 2026Article
- Tke5 is a Pseudomonas putida toxin that kills plant pathogens by depolarising membranes.Communications biology · 2026Article
- Structural insights into the antibacterial function of the Pseudomonas putida effector Tke5.The EMBO journal · 2026Article
- Exploring the computing power of microbes that shapes the environment.Current opinion in microbiology · 2026Review
- The glucose uptake inhibitor SgrS is induced by D-serine yet does not contribute to growth arrest in enterohaemorrhagicMicrobiology (Reading, England) · 2026Article
- Dynamics of bacterial biofilm development imaged using light sheet fluorescence microscopy.Biochemistry and biophysics reports · 2025Article
- Why cellular computations challenge our design principles.Seminars in cell & developmental biology · 2025Review
- Digitizing the Blue Light-Activated T7 RNA Polymerase System with aACS synthetic biology · 2025Article
- Genetic designs for stochastic and probabilistic biocomputing.Physical review. E · 2025Article
- Programming Nutrient Detection with Modular Regulators for Dynamic Control of Microbial Biosynthesis.ACS synthetic biology · 2025Article
- Engineering a phi15-based expression system for stringent gene expression in Pseudomonas putida.Communications biology · 2025Article
- AND Logic Based on Suppressor tRNAs Enables Stringent Control of Sliding Base Editors inACS synthetic biology · 2024Article
- A dual-inducible control system for multistep biosynthetic pathways.Journal of biological engineering · 2024Article
- Optimized CRISPR Interference System for InvestigatingACS synthetic biology · 2024Article
- Emergent digital bio-computation through spatial diffusion and engineered bacteria.Nature communications · 2024Article
- Long-duration environmental biosensing by recording analyte detection in DNA using recombinase memory.Applied and environmental microbiology · 2024Article
- GenoMine: a CRISPR-Cas9-based kill switch for biocontainment ofFrontiers in bioengineering and biotechnology · 2024Article
- Unlocking the strength of inducible promoters in Gram-negative bacteria.Microbial biotechnology · 2023Article
- An electrogenetic toggle switch model.Microbial biotechnology · 2023Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
While prokaryotic promoters controlled by signal-responding regulators typically display a range of input/output ratios when exposed to cognate inducers, virtually no naturally occurring cases are known to have an OFF state of zero transcription-as ideally needed for synthetic circuits. To overcome this problem, we have modelled and implemented a simple digitalizer module that completely suppresses the basal level of otherwise strong promoters in such a way that expression in the absence of induction is entirely impeded. The circuit involves the interplay of a translation-inhibitory sRNA with the translational coupling of the gene of interest to a repressor such as LacI. The digitalizer module was validated with the strong inducible promoters Pm (induced by XylS in the presence of benzoate) and PalkB (induced by AlkS/dicyclopropyl ketone) and shown to perform effectively in both Escherichia coli and the soil bacterium Pseudomonas putida. The distinct expression architecture allowed cloning and conditional expression of, e.g. colicin E3, one molecule of which per cell suffices to kill the host bacterium. Revertants that escaped ColE3 killing were not found in hosts devoid of insertion sequences, suggesting that mobile elements are a major source of circuit inactivation in vivo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.