ArticleMolecular systems biology2019
Measuring glycolytic flux in single yeast cells with an orthogonal synthetic biosensor.
Article in Molecular systems biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Translational antagonism enables bet-hedging in yeast via mitochondrial bistability.bioRxiv : the preprint server for biology · 2026Article
- Cell cycle-dependent protein dynamics in budding yeast resolved by deconvolution of bulk proteomics.Molecular systems biology · 2026Article
- The return of metabolism: biochemistry and physiology of glycolysis.Biological reviews of the Cambridge Philosophical Society · 2026Review
- Single cell profiling framework reveals metabolic subpopulations as drivers of bioproduction heterogeneity.Nature communications · 2025Article
- The lives of cells, recorded.Nature reviews. Genetics · 2025Review
- Enhanced cellular longevity arising from environmental fluctuations.Cell systems · 2024Article
- Article
- Probing Single-Cell Fermentation Fluxes and Exchange Networks via pH-Sensing Hybrid Nanofibers.ACS nano · 2023Article
- Temporal segregation of biosynthetic processes is responsible for metabolic oscillations during the budding yeast cell cycle.Nature metabolism · 2023Article
- Two-way communication between cell cycle and metabolism in budding yeast: what do we know?Frontiers in microbiology · 2023Article
- Monitoring glycolytic dynamics in single cells using a fluorescent biosensor for fructose 1,6-bisphosphate.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Visualizing the pH in Escherichia coli Colonies via the Sensor Protein mCherryEA Allows High-Throughput Screening of Mutant Libraries.mSystems · 2022Article
- Engineering of Synthetic Transcriptional Switches in Yeast.Life (Basel, Switzerland) · 2022Review
- On the design principles of metabolic flux sensing.Biophysical journal · 2022Article
- Biosensor for branched-chain amino acid metabolism in yeast and applications in isobutanol and isopentanol production.Nature communications · 2022Article
- From imaging a single cell to implementing precision medicine: an exciting new era.Emerging topics in life sciences · 2021Article
- Reversible amyloids of pyruvate kinase couple cell metabolism and stress granule disassembly.Nature cell biology · 2021Article
- Physical bioenergetics: Energy fluxes, budgets, and constraints in cells.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Single-cell image analysis to explore cell-to-cell heterogeneity in isogenic populations.Cell systems · 2021Review
- Single-Cell Technologies to Understand the Mechanisms of Cellular Adaptation in Chemostats.Frontiers in bioengineering and biotechnology · 2020Article
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metabolic heterogeneity between individual cells of a population harbors significant challenges for fundamental and applied research. Identifying metabolic heterogeneity and investigating its emergence require tools to zoom into metabolism of individual cells. While methods exist to measure metabolite levels in single cells, we lack capability to measure metabolic flux, i.e., the ultimate functional output of metabolic activity, on the single-cell level. Here, combining promoter engineering, computational protein design, biochemical methods, proteomics, and metabolomics, we developed a biosensor to measure glycolytic flux in single yeast cells. Therefore, drawing on the robust cell-intrinsic correlation between glycolytic flux and levels of fructose-1,6-bisphosphate (FBP), we transplanted the B. subtilis FBP-binding transcription factor CggR into yeast. With the developed biosensor, we robustly identified cell subpopulations with different FBP levels in mixed cultures, when subjected to flow cytometry and microscopy. Employing microfluidics, we were also able to assess the temporal FBP/glycolytic flux dynamics during the cell cycle. We anticipate that our biosensor will become a valuable tool to identify and study metabolic heterogeneity in cell populations.
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