ArticleCell2020
In Situ Structure of an Intact Lipopolysaccharide-Bound Bacterial Surface Layer.
Article in Cell, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
50 citing papers in PubMed, 111 citations in OpenAlex.
- Prolific S-layer shedding and associated proteins from the methanotrophApplied and environmental microbiology · 2026Article
- Subcellular chemical mapping using correlated cryogenic electron and mass spectrometry imaging.Nature methods · 2026Article
- Automated assembly of protein complexes from cryo-EM maps with structure-informed Monte Carlo Tree Search.bioRxiv : the preprint server for biology · 2026Article
- S-layer architecture and glycosylation and their role in shaping virus-host specificity in prokaryotes.Frontiers in microbiology · 2026Review
- Cryo-electron tomography reveals OmpB is required for themicroPublication biology · 2026Article
- Hybrid Detergents Facilitate Scalable Charge Reduction and Stabilize Membrane Proteins While Retaining Lipid Interactions.Chemistry (Weinheim an der Bergstrasse, Germany) · 2025Article
- Cryo-EM structure and polar assembly of the PS2 S-layer ofProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Outer Membrane Vesicles fromACS nano · 2025Article
- A new age in structural S-layer biology: Experimental and in silico milestones.The Journal of biological chemistry · 2025Review
- Crystal structure of the C1 domain of the surface-layer protein SlpM from Lactobacillus brevis: a module involved in protein self-assembly.Acta crystallographica. Section F, Structural biology communications · 2025Article
- Mapping the ultrastructural topology of the corynebacterial cell surface.PLoS biology · 2025Article
- CryoSamba: Self-supervised deep volumetric denoising for cryo-electron tomography data.Journal of structural biology · 2025Article
- Microbial hauberks: composition and function of surface layer proteins in gammaproteobacterial methanotrophs.Applied and environmental microbiology · 2025Article
- Structural biology inside multicellular specimens using electron cryotomography.Quarterly reviews of biophysics · 2025Review
- Architecture of the Sap S-layer ofProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Composition and in situ structure of theScience advances · 2024Article
- Cohesive Living Bacterial Films with Tunable Mechanical Properties from Cell Surface Protein Display.ACS synthetic biology · 2024Article
- The parasitic lifestyle of an archaeal symbiont.Nature communications · 2024Article
- Lifecycle of a predatory bacterium vampirizing its prey through the cell envelope and S-layer.Nature communications · 2024Article
- Cell cycle dependent coordination of surface layer biogenesis in Caulobacter crescentus.Nature communications · 2024Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
Abstract
Most bacterial and all archaeal cells are encapsulated by a paracrystalline, protective, and cell-shape-determining proteinaceous surface layer (S-layer). On Gram-negative bacteria, S-layers are anchored to cells via lipopolysaccharide. Here, we report an electron cryomicroscopy structure of the Caulobacter crescentus S-layer bound to the O-antigen of lipopolysaccharide. Using native mass spectrometry and molecular dynamics simulations, we deduce the length of the O-antigen on cells and show how lipopolysaccharide binding and S-layer assembly is regulated by calcium. Finally, we present a near-atomic resolution in situ structure of the complete S-layer using cellular electron cryotomography, showing S-layer arrangement at the tip of the O-antigen. A complete atomic structure of the S-layer shows the power of cellular tomography for in situ structural biology and sheds light on a very abundant class of self-assembling molecules with important roles in prokaryotic physiology with marked potential for synthetic biology and surface-display applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.