Evidence map›Paper›PMID 31883794›Full record

ArticleCell2020

A Patient-Derived Glioblastoma Organoid Model and Biobank Recapitulates Inter- and Intra-tumoral Heterogeneity.

Fadi Jacob, Ryan D Salinas, Daniel Y Zhang, Phuong T T Nguyen, Jordan G Schnoll, Samuel Zheng Hao Wong, Radhika Thokala, Saad Sheikh, Deeksha Saxena, Stefan Prokop and 13 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 696 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
696citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

696 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Review
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Review
  18. Review
  19. Review
  20. Article

636 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

23 authors.

Fadi JacobDepartment of Neuroscience and Mahoney Institute for Neurosciences, University of Pennsylvania, Philadelphia, PA 19104, USA; The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Medical Scientist Training Program, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Ryan D SalinasDepartment of Neurosurgery, University of Pennsylvania, Philadelphia, PA 19104, USA.
Daniel Y ZhangBiochemistry and Molecular Biophysics Graduate Group, University of Pennsylvania, Philadelphia, PA 19104, USA.
Phuong T T NguyenNeuroscience Graduate Group, University of Pennsylvania, Philadelphia, PA 19104, USA.
Jordan G SchnollDepartment of Neuroscience and Mahoney Institute for Neurosciences, University of Pennsylvania, Philadelphia, PA 19104, USA.
Samuel Zheng Hao WongDepartment of Neuroscience and Mahoney Institute for Neurosciences, University of Pennsylvania, Philadelphia, PA 19104, USA; Graduate Program in Cellular and Molecular Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Radhika ThokalaDepartment of Neurosurgery, University of Pennsylvania, Philadelphia, PA 19104, USA.
Saad SheikhDepartment of Radiation Oncology, University of Pennsylvania, Philadelphia, PA 19104, USA.
Deeksha SaxenaDepartment of Radiation Oncology, University of Pennsylvania, Philadelphia, PA 19104, USA.
Stefan ProkopDepartment of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Di-Ao LiuDepartment of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA.
Xuyu QianDepartment of Neuroscience and Mahoney Institute for Neurosciences, University of Pennsylvania, Philadelphia, PA 19104, USA; Bioengineering Graduate Program, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Dmitriy PetrovDepartment of Neurosurgery, University of Pennsylvania, Philadelphia, PA 19104, USA.
Timothy LucasDepartment of Neurosurgery, University of Pennsylvania, Philadelphia, PA 19104, USA.
H Isaac ChenDepartment of Neurosurgery, University of Pennsylvania, Philadelphia, PA 19104, USA; Institute for Regenerative Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA; Corporal Michael J. Crescenz Veterans Affairs Medical Center, Philadelphia, PA 19104, USA.
Jay F DorseyDepartment of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA; Glioblastoma Translational Center of Excellence, The Abramson Cancer Center, University of Pennsylvania, Philadelphia, PA 19104, USA.
Kimberly M ChristianDepartment of Neuroscience and Mahoney Institute for Neurosciences, University of Pennsylvania, Philadelphia, PA 19104, USA.
Zev A BinderDepartment of Neurosurgery, University of Pennsylvania, Philadelphia, PA 19104, USA; Glioblastoma Translational Center of Excellence, The Abramson Cancer Center, University of Pennsylvania, Philadelphia, PA 19104, USA.
MacLean NasrallahDepartment of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA; Glioblastoma Translational Center of Excellence, The Abramson Cancer Center, University of Pennsylvania, Philadelphia, PA 19104, USA.
Steven BremDepartment of Neurosurgery, University of Pennsylvania, Philadelphia, PA 19104, USA; Glioblastoma Translational Center of Excellence, The Abramson Cancer Center, University of Pennsylvania, Philadelphia, PA 19104, USA.
Donald M O'RourkeDepartment of Neurosurgery, University of Pennsylvania, Philadelphia, PA 19104, USA; Glioblastoma Translational Center of Excellence, The Abramson Cancer Center, University of Pennsylvania, Philadelphia, PA 19104, USA. Electronic address: donald.orourke@pennmedicine.upenn.edu.
Guo-Li MingDepartment of Neuroscience and Mahoney Institute for Neurosciences, University of Pennsylvania, Philadelphia, PA 19104, USA; Institute for Regenerative Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA; Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, PA 19104, USA; Department of Psychiatry, University of Pennsylvania, Philadelphia, PA 19104, USA. Electronic address: ming@pennmedicine.upenn.edu.
Hongjun SongDepartment of Neuroscience and Mahoney Institute for Neurosciences, University of Pennsylvania, Philadelphia, PA 19104, USA; Institute for Regenerative Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA; Glioblastoma Translational Center of Excellence, The Abramson Cancer Center, University of Pennsylvania, Philadelphia, PA 19104, USA; Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, PA 19104, USA. Electronic address: shongjun@pennmedicine.upenn.edu.

Funding

Modeling Zika virus pathogenesis and potential intervention during neural development using a human brain organoid systemU19AI131130 · NIAID · UNIVERSITY OF PENNSYLVANIA · PI BRINTON, MARGO A · 2017 to 2021
$7.7M
Continuous Neurogenesis in the Mammalian HippocampusR35NS116843 · NINDS · UNIVERSITY OF PENNSYLVANIA · PI HONGJUN SONG · 2020 to 2026
$6.9M
Functional roles of genetic risk factors for brain disorders in neurogenesis and neurodevelopmentR35NS097370 · NINDS · UNIVERSITY OF PENNSYLVANIA · PI MING, GUO-LI · 2017 to 2024
$6.7M
Local circuitry mechanisms regulating adult hippocampal neurogenesisR37NS047344 · NINDS · UNIVERSITY OF PENNSYLVANIA · PI SONG, HONGJUN · 2013 to 2019
$2.9M
Radiation and Receptor Targeted RadioTheranostic Nanoparticles for GlioblastomaR01CA181429 · NCI · UNIVERSITY OF PENNSYLVANIA · PI DORSEY, JAY FITZGERALD · 2014 to 2018
$2.4M
NCI NIH HHS R01 CA181429NIAID NIH HHS U19 AI131130NINDS NIH HHS R35 NS097370NINDS NIH HHS R35 NS116843NINDS NIH HHS R37 NS047344
6 · The paper itself

Abstract

Glioblastomas exhibit vast inter- and intra-tumoral heterogeneity, complicating the development of effective therapeutic strategies. Current in vitro models are limited in preserving the cellular and mutational diversity of parental tumors and require a prolonged generation time. Here, we report methods for generating and biobanking patient-derived glioblastoma organoids (GBOs) that recapitulate the histological features, cellular diversity, gene expression, and mutational profiles of their corresponding parental tumors. GBOs can be generated quickly with high reliability and exhibit rapid, aggressive infiltration when transplanted into adult rodent brains. We further demonstrate the utility of GBOs to test personalized therapies by correlating GBO mutational profiles with responses to specific drugs and by modeling chimeric antigen receptor T cell immunotherapy. Our studies show that GBOs maintain many key features of glioblastomas and can be rapidly deployed to investigate patient-specific treatment strategies. Additionally, our live biobank establishes a rich resource for basic and translational glioblastoma research.

Indexed as

AdultAgedAged, 80 and overAnimalsBiological Specimen BanksCell Culture TechniquesFemaleGlioblastomaHumansMaleMiceMice, NudeMiddle AgedModels, BiologicalOrganoidsReproducibility of Resultsbiobankcancer modelingCAR-T cellsdrug testingglioblastomaOrganoidpersonalized therapiestranslationaltumor heterogeneityxenograft

Identifiers

PMID31883794
PMCPMC7556703

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.