ArticleMethods in molecular biology (Clifton, N.J.)2020
Metabolite, Protein, and Lipid Extraction (MPLEx): A Method that Simultaneously Inactivates Middle East Respiratory Syndrome Coronavirus and Allows Analysis of Multiple Host Cell Components Following Infection.
Article in Methods in molecular biology (Clifton, N.J.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- The Specific Carbohydrate Diet and Diet Modification as Induction Therapy for Pediatric Crohn's Disease: A Randomized Diet Controlled Trial.Nutrients · 2020Trial
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- Adapting a commercial sample extraction protocol for biosafety level 3/4 compatible plasma metabolomics analysis.Journal of mass spectrometry and advances in the clinical lab · 2025Article
- Evaluation of exploratory fluid biomarkers from a phase 1 senolytic trial in mild Alzheimer's disease.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2025Article
- Too dim, too bright, and just right: Systems analysis of the Chlamydomonas diurnal program under limiting and excess light.The Plant cell · 2025Article
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- Progress in multi-omics studies of osteoarthritis.Biomarker research · 2025Review
- Metabolomic and transcriptomic remodeling of bone marrow myeloid cells in response to maternal obesity.American journal of physiology. Endocrinology and metabolism · 2025Article
- A compendium of multi-omics data illuminating host responses to lethal human virus infections.Scientific data · 2024Article
- Multi-omics of NET formation and correlations with CNDP1, PSPB, and L-cystine levels in severe and mild COVID-19 infections.Heliyon · 2023Article
- Prior metabolite extraction fully preserves RNAseq quality and enables integrative multi-'omics analysis of the liver metabolic response to viral infection.RNA biology · 2023Article
- The role of lipids in the pathophysiology of coronavirus infections.Osong public health and research perspectives · 2021Article
- Lipidome is lipids regulator in gastrointestinal tract and it is a life collar in COVID-19: A review.World journal of gastroenterology · 2021Review
- Mass Spectrometry-based Proteomics and Glycoproteomics in COVID-19 Biomarkers Identification: A Mini-review.Journal of analysis and testing · 2021Review
- Media matters! Alterations in the loading and release of Histoplasma capsulatum extracellular vesicles in response to different nutritional milieus.Cellular microbiology · 2020Article
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Authors and funding
8 authors.
Funding
Abstract
Mass spectrometry (MS)-based, integrated proteomics, metabolomics, and lipidomics (collectively, multi-omics) studies provide a very detailed snapshot of virus-induced changes to the host following infection and can lead to the identification of novel prophylactic and therapeutic targets for preventing or lessening disease severity. Multi-omics studies with Middle East respiratory syndrome coronavirus (MERS-CoV) are challenging as the requirements of biosafety level 3 containment limit the numbers of samples that can be safely managed. To address these issues, the multi-omics sample preparation technique MPLEx (metabolite, protein, and lipid extraction) was developed to partition a single sample into three distinct parts (metabolites, proteins, and lipids) for multi-omics analysis, while simultaneously inactivating MERS-CoV by solubilizing and disrupting the viral envelope and denaturing viral proteins. Here we describe the MPLEx protocol, highlight the step of inactivation, and describe the details of downstream processing, instrumental analysis of the three separate analytes, and their subsequent informatics pipelines.
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