ArticleMethods in molecular biology (Clifton, N.J.)2020
Evaluation of MERS-CoV Neutralizing Antibodies in Sera Using Live Virus Microneutralization Assay.
Article in Methods in molecular biology (Clifton, N.J.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 43 citations in OpenAlex.
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- Cross-Species Surveillance of Respiratory Viruses in Domestic and Wild Mammals of an Urban Atlantic Forest from Brazil.EcoHealth · 2025Article
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- Research progress in methods for detecting neutralizing antibodies against SARS-CoV-2.Analytical biochemistry · 2023Review
- A new screening system for entry inhibitors based on cell-to-cell transmitted syncytia formation mediated by self-propagating hybrid VEEV-SARS-CoV-2 replicon.Emerging microbes & infections · 2022Article
- Article
- Predictive factors of a viral neutralizing humoral response after a third dose of COVID-19 mRNA vaccine.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2022Article
- Can anti-parasitic drugs help control COVID-19?Future virology · 2022Review
- Reduced levels of convalescent neutralizing antibodies against SARS-CoV-2 B.1+L249S+E484K lineage.Virus research · 2022Article
- Persistence of Anti-SARS-CoV-2 Spike IgG Antibodies Following COVID-19 Vaccines.Infection and drug resistance · 2022Article
- In-depth Characterization of Vaccine Breakthrough Infections With SARS-CoV-2 Among Health Care Workers in a Dutch Academic Medical Center.Open forum infectious diseases · 2022Article
- Recent Developments in SARS-CoV-2 Neutralizing Antibody Detection Methods.Current medical science · 2021Review
- Comparison of SARS-CoV-2 neutralizing antibody testing of convalescent plasma donations in the Netherlands and England: A pilot study.Health science reports · 2021Article
- A class II MHC-targeted vaccine elicits immunity against SARS-CoV-2 and its variants.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Roles of existing drug and drug targets for COVID-19 management.Metabolism open · 2021Article
- COVID-19 convalescent plasma as long-term therapy in immunodeficient patients?Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine · 2021Article
- Ad26.COV2.S protects Syrian hamsters against G614 spike variant SARS-CoV-2 and does not enhance respiratory disease.NPJ vaccines · 2021Article
- Recombinant SARS-CoV-2 RBD with a built in T helper epitope induces strong neutralization antibody response.Vaccine · 2021Article
- Dynamics of antibodies to SARS-CoV-2 in convalescent plasma donors.Clinical & translational immunology · 2021Article
- Investigation of SARS-CoV-2 infection in dogs and cats of humans diagnosed with COVID-19 in Rio de Janeiro, Brazil.PloS one · 2021Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The microneutralization (MN) assay is a standard and important technique in virology, immunology, and epidemiology. It is a highly specific and sensitive assay for evaluating virus-specific neutralizing antibodies (nAbs) in human and animal sera. It provides the most precise answer to whether or not an individual or animal has antibodies that can neutralize or inhibit the infectivity of a specific virus strain. However, using live virus-based MN assay might require working under high containment facilities especially when dealing with high-risk pathogens such as the Middle East respiratory syndrome-coronavirus (MERS-CoV). In this chapter, we describe the isolation, amplification, and titration of MERS-CoV, as well as detailed MN assay to measure nAb levels in sera from different mammalian species.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.