ArticleScientific reports2019
Stepped vitrification technique for human ovarian tissue cryopreservation.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 54 citations in OpenAlex.
- Rapamycin preserves primordial follicles during closed‑system vitrification of mouse ovarian tissue.Clinical and experimental reproductive medicine · 2026Article
- Technological Advances of Cryopreservation in Ovarian Tissue for Female Children: Exploring the Molecular Insights and Mechanisms.International journal of molecular sciences · 2026Review
- Fertility under fire: how chemotherapy harms the ovaries and the science fighting back?Journal of ovarian research · 2026Review
- Scalable Purification of Iron Oxide Nanoparticles for Organ Cryopreservation and Transplantation.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- The effect of cryoprotectant on the quality of post-warming goat oocytes by examining SOD levels.Open veterinary journal · 2025Article
- Primordial Follicle Response to Two Methods of Ovarian Cortex Retrieval and Vitrification: A Pilot Study.Cureus · 2025Article
- Evaluation of Different Cryoprotectant Combinations in Vitrification and Slow Freezing for Ovarian Tissue Preservation in Domestic Cats.Reproduction in domestic animals = Zuchthygiene · 2025Article
- Vitrification alters growth differentiation factor 9 and follicle-stimulating hormone receptor expression in human cumulus-mural granulosa cells.Clinical and experimental reproductive medicine · 2024Article
- Review
- Exploring the knowledge, attitudes, and perceptions of women of reproductive age towards fertility and elective oocyte cryopreservation for age-related fertility decline in the UK: a cross-sectional survey.Human reproduction (Oxford, England) · 2023Article
- Accelerating cryoprotectant delivery using vacuum infiltration.Cryobiology · 2023Article
- Drastic Loss of Antral Follicles Due to Gene Expression Dysregulation Occurs on the First Day After Subcutaneous Ovarian Transplantation.Reproductive sciences (Thousand Oaks, Calif.) · 2023Article
- Sound waves for solving the problem of recrystallization in cryopreservation.Scientific reports · 2023Article
- A comprehensive review and update on human fertility cryopreservation methods and tools.Frontiers in veterinary science · 2023Review
- Systematic review and meta-analysis on patented and non-patented vitrification processes to ovarian tissue reported between 2000 and 2021.Animal reproduction · 2023Review
- Article
- Age-related fertility decline: is there a role for elective ovarian tissue cryopreservation?Human reproduction (Oxford, England) · 2022Review
- Ovarian Stem Cells (OSCs) from the Cryopreserved Ovarian Cortex: A Potential for Neo-Oogenesis in Women with Cancer-Treatment Related Infertility: A Case Report and a Review of Literature.Current issues in molecular biology · 2022Review
- The effect of different vitrification protocols on cell survival in human ovarian tissue: a pilot study.Journal of ovarian research · 2021Article
- Equine ovarian tissue xenografting: impacts of cooling, vitrification, and VEGF.Reproduction & fertility · 2021Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 4 countries.
Funding
Abstract
The advantage of stepped vitrification (SV) is avoiding ice crystal nucleation, while decreasing the toxic effects of high cryoprotectant concentrations. We aimed to test this method for human ovarian tissue cryopreservation. Ovarian cortex was taken from 7 fertile adult women. Samples were subjected to an SV protocol performed in an automatic freezer, which allowed sample transfer to ever higher concentrations of dimethyl sulfoxide (DMSO) as the temperature was reduced. Histological evaluation of the vitrified-warmed tissue showed large numbers of degenerated follicles after 24 hours of in vitro culture. We therefore evaluated DMSO perfusion rates by X-ray computed tomography, ice crystal formation by freeze-substitution, and cell toxicity by transmission electron microscopy, seeking possible reasons why follicles degenerated. Although cryoprotectant perfusion was considered normal and no ice crystals were formed in the tissue, ultrastructural analysis detected typical signs of DMSO toxicity, such as mitochondria degeneration, alterations in chromatin condensation, cell vacuolization and extracellular matrix swelling in both stromal and follicular cells. The findings indicated that the method failed to preserve follicles due to the high concentrations of DMSO used. However, adaptations can be made to avoid toxicity to follicles caused by elevated levels of cryoprotectants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.