ArticleScientific reports2019
A Biochemiluminescent Sialidase Assay for Diagnosis of Bacterial Vaginosis.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.
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Who cites it
11 citing papers in PubMed, 1 synthesis or guideline pooled it, 27 citations in OpenAlex.
- BVBlue as a diagnostic instrument for the diagnosis of bacterial vaginosis: a systematic review.BMC women's health · 2025Pooled it
- Advances in biomonitoring technologies for women's health.Nature communications · 2025Review
- Rapid diagnosis of bacterial vaginosis using machine-learning-assisted surface-enhanced Raman spectroscopy of human vaginal fluids.mSystems · 2025Article
- The role of sialidases in the pathogenesis of bacterial vaginosis and their use as a promising pharmacological target in bacterial vaginosis.Frontiers in cellular and infection microbiology · 2024Review
- Review
- Effect of a combination of pea protein, grape seed extract and lactic acid in an in vivo model of bacterial vaginosis.Scientific reports · 2023Article
- Association of Dietary Glycemic Index, Glycemic Load, Insulin Index, and Insulin Load with Bacterial Vaginosis in Iranian Women: A Case-Control Study.Infectious diseases in obstetrics and gynecology · 2022Article
- Vaginal sialoglycan foraging by Gardnerella vaginalis: mucus barriers as a meal for unwelcome guests?Glycobiology · 2021Review
- Nanophotonic Sialidase Immunoassay for Bacterial Vaginosis Diagnosis.ACS pharmacology & translational science · 2021Article
- Sialidase substrates for Sialdiase assays - activity, specificity, quantification and inhibition.Glycoconjugate journal · 2020Review
- Modified Enzyme Substrates for the Detection of Bacteria: A Review.Molecules (Basel, Switzerland) · 2020Review
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Authors and funding
13 authors at 6 institutions in 3 countries.
Funding
Abstract
Bacterial vaginosis (BV) is a common condition among women of reproductive age. A sensitive, quantitative and rapid assay is needed for the diagnosis of and, particularly, therapy monitoring for BV. Bacterial sialidase appears to play an important role in bacterial biofilms on vaginal epithelium, a condition closely associated with BV. Here, we report a biochemiluminescent sialidase assay that uses a substrate derivatized with firefly luciferin. In the presence of sialidase in the reaction, the substrate is cleaved to release luciferin, which is subsequently oxidized by firefly luciferase to generate a light signal. Thus, the light signal intensity can be used to detect and measure the relative concentration of sialidase in a vaginal sample as a means of BV diagnosis. All reagents are present in a reagent bead and sample buffer, enabling essentially a one-step assay. The assay is highly sensitive and quantitative, with a sensitivity and specificity of 95.40% and 94.94%, respectively, compared to the Amsel method. Interestingly, only 27.6% of those with BV had high levels of sialidase activity with a signal to cutoff ratio of 10 or more. The assay may be used for diagnosis of BV, risk assessment of BV patients in terms of sialidase activity levels, and monitoring antibiotic therapy.
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