ArticleScientific reports2019
An optimized, robust and reproducible protocol to generate well-differentiated primary nasal epithelial models from extremely premature infants.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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6 citing papers in PubMed, 10 citations in OpenAlex.
- Respiratory Syncytial Virus Nonstructural Protein 1 Inhibits Production of Cytokines and Chemokines by Differentiated Primary Nasal Epithelial Cells Cultured at Air-Liquid Interface.Journal of innate immunity · 2026Article
- Theratyping as a tool to guide clinical decision-making in patients with suspected cystic fibrosis and variants currently ineligible for CFTR modulator therapy.Respiratory medicine · 2025Article
- A primary cell model of the very preterm epithelium reveals barrier defects at 1 year of age.ERJ open research · 2025Article
- Bacterial extracellular vesicles as intranasal postbiotics: Detailed characterization and interaction with airway cells.Journal of extracellular vesicles · 2024Article
- A clinical protocol for a German birth cohort study of the Maturation of Immunity Against respiratory viral Infections (MIAI).Frontiers in immunology · 2024Article
- Prematurity alters the progenitor cell program of the upper respiratory tract of neonates.Scientific reports · 2021Article
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Authors and funding
12 authors at 2 institutions in 3 countries.
Funding
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Abstract
Extremely premature infants are prone to severe respiratory infections, and the mechanisms underlying this exceptional susceptibility are largely unknown. Nasal epithelial cells (NEC) represent the first-line of defense and adult-derived ALI cell culture models show promising results in mimicking in vivo physiology. Therefore, the aim of this study was to develop a robust and reliable protocol for generating well-differentiated cell culture models from NECs of extremely premature infants. Nasal brushing was performed in 13 extremely premature infants at term corrected age and in 11 healthy adult controls to obtain NECs for differentiation at air-liquid interface (ALI). Differentiation was verified using imaging and functional analysis. Successful isolation and differentiation was achieved for 5 (38.5%) preterm and 5 (45.5%) adult samples. Preterm and adult ALI-cultures both showed well-differentiated morphology and ciliary function, however, preterm cultures required significantly longer cultivation times for acquiring full differentiation (44 ± 3.92 vs. 23 ± 1.83 days; p < 0.0001). Moreover, we observed that recent respiratory support may impair successful NECs isolation. Herewithin, we describe a safe, reliable and reproducible method to generate well-differentiated ALI-models from NECs of extremely premature infants. These models provide a valuable foundation for further studies regarding immunological and inflammatory responses and respiratory disorders in extremely premature infants.
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