ArticleScientific reports2019
Detection and phenotyping of extracellular vesicles by size exclusion chromatography coupled with on-line fluorescence detection.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 38 citations in OpenAlex.
- Low-MESF fluorescent reference particles for flow cytometry analysis of extracellular vesicles.Scientific reports · 2026Article
- Production and Purification of Mesenchymal Stem Cell-Derived Extracellular Vesicles Using Preferential Exclusion Chromatography.Electrophoresis · 2026Article
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- Anti-fibrotic effect ofFrontiers in bioengineering and biotechnology · 2026Article
- Comprehensive Characterisation of Extracellular Vesicle Preparations Using Multiparametric Size-Exclusion Chromatography.International journal of molecular sciences · 2025Article
- Effects of electric fields on the release and content of extracellular vesicles.Journal of extracellular biology · 2024Article
- Inline Raman Spectroscopy Provides Versatile Molecular Monitoring for Platelet Extracellular Vesicle Purification with Anion-Exchange Chromatography.International journal of molecular sciences · 2024Article
- Molecular imaging of bacterial outer membrane vesicles based on bacterial surface display.Scientific reports · 2023Article
- Removal and identification of external protein corona members from RBC-derived extracellular vesicles by surface manipulating antimicrobial peptides.Journal of extracellular biology · 2023Article
- Exosomes and Their Bioengineering Strategies in the Cutaneous Wound Healing and Related Complications: Current Knowledge and Future Perspectives.International journal of biological sciences · 2023Review
- Advances in Therapeutic Applications of Extracellular Vesicles.International journal of nanomedicine · 2023Review
- Article
- Introduction of Nanomaterials to Biosensors for Exosome Detection: Case Study for Cancer Analysis.Biosensors · 2022Review
- Storage conditions determine the characteristics of red blood cell derived extracellular vesicles.Scientific reports · 2022Article
- Storage conditions determine the characteristics of red blood cell derived extracellular vesicles.Scientific reports · 2022Article
- Comparative Analysis of Platelet-Derived Extracellular Vesicles Using Flow Cytometry and Nanoparticle Tracking Analysis.International journal of molecular sciences · 2021Article
- A call for Rigor and standardization in plant extracellular vesicle research.Journal of extracellular vesicles · 2021Article
- Electromagnetic Piezoelectric Acoustic Sensor Detection of Extracellular Vesicles through Interaction with Detached Vesicle Proteins.Biosensors · 2020Article
- A Review of Exosomal Isolation Methods: Is Size Exclusion Chromatography the Best Option?International journal of molecular sciences · 2020Review
- Cholera Toxin Subunit B for Sensitive and Rapid Determination of Exosomes by Gel Filtration.Membranes · 2020Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
New methods for quantifying extracellular vesicles (EVs) in complex biofluids are critically needed. We report the development of a new technology combining size exclusion chromatography (SEC), a commonly used EV purification technique, with fluorescence detection of specifically labelled EVs. The resulting platform, Flu-SEC, demonstrates a linear response to concentration of specific EVs and could form the basis of a system with phenotyping capability. Flu-SEC was validated using red blood cell derived EVs (REVs), which provide an ideal EV model with monodisperse size distribution and high EV concentration. Microfluidic Resistive Pulse Sensing (MRPS) was used to accurately determine the size distribution and concentration of REVs. Anti-CD235a antibody, specific to glycophorin A, and the more general wheat germ agglutinin (WGA), were selected to label REVs. The results show the quantitative power of Flu-SEC: a highly linear fluorescence response over a wide range of concentrations. Moreover, the Flu-SEC technique reports the ratio of EV-bound and free-antibody molecules, an important metric for determining optimal labelling conditions for other applications. Flu-SEC represents an orthogonal tool to single-particle fluorescent methods such as flow cytometry and fluorescent NTA, for the quantification and phenotyping of EVs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.