ArticleScientific reports2019
Selection of Reference Genes for qPCR Analyses of Gene Expression in Ramie Leaves and Roots across Eleven Abiotic/Biotic Treatments.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed, 50 citations in OpenAlex.
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- qRT-PCR Reference Gene Selection for the Discoloration of Tender Leaves in Hawk Tea (Current issues in molecular biology · 2025Article
- Cross-species stability of reference genes in medicinal plants Arnica montana and Arnica chamissonis.Scientific reports · 2024Article
- Development of a Reference Transcriptome and Identification of Differentially Expressed Genes Linked to Salt Stress in Salt Marsh Grass (Plants (Basel, Switzerland) · 2024Article
- Identification and validation of stable reference genes for RT-qPCR analyses of Kobresia littledalei seedlings.BMC plant biology · 2024Article
- Reference genes for Eucalyptus spp. under Beauveria bassiana inoculation and subsequently infestation by the galling wasp Leptocybe invasa.Scientific reports · 2024Article
- How to explore what is hidden? A review of techniques for vascular tissue expression profile analysis.Plant methods · 2023Review
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- Comparative transcriptome analysis of Saposhnikovia divaricata to reveal drought and rehydration adaption strategies.Molecular biology reports · 2023Article
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- Selection of suitable candidate genes for mRNA expression normalization in bulbil development of Pinellia ternata.Scientific reports · 2022Article
- Transcriptomic Analysis Suggests Auxin Regulation in Dorsal-Ventral Petal Asymmetry of Wild ProgenitorInternational journal of molecular sciences · 2022Article
- Gene Expression Profiles Suggest a Better Cold Acclimation of Polyploids in the Alpine SpeciesGenes · 2021Article
- Selection and Validation of Reference Genes for RT-qPCR Analysis inInternational journal of molecular sciences · 2021Article
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- Reference gene selection for qRT-PCR analyses of luffa (Luffa cylindrica) plants under abiotic stress conditions.Scientific reports · 2021Article
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Authors and funding
13 authors at 2 institutions in 2 countries.
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Abstract
Quantitative real-time PCR (qPCR) is commonly used for deciphering gene functions. For effective qPCR analyses, suitable reference genes are needed for normalization. The objective of this study is to identify the appropriate reference gene(s) for qPCR analyses of the leaves and roots of ramie (Boehmeria nivea L.), an important natural fiber crop. To accomplish this goal, we investigated the expression patterns of eight common plant qPCR reference genes in ramie leaves and roots under five abiotic stresses, five hormonal treatments, and one biotic stress. The relative expression stabilities of the eight genes were evaluated using four common but different approaches: geNorm, NormFinder, BestKeeper, and RefFinder. Across the 11 tested conditions, ACT1 was the most stably expressed among the eight genes while GAPDH displayed the biggest variation. Overall, while variations in the suggested reference genes were found for different tissue x treatment combinations, our analyses revealed that together, genes ACT1, CYP2, and UBQ can provide robust references for gene expression studies of ramie leaves under most conditions, while genes EF-1α, TUB, and ACT1 can be used for similar studies of ramie roots. Our results should help future functional studies of the genes in ramie genome across tissues and environmental conditions.
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