ArticleScientific reports2019
High-frequency random DNA insertions upon co-delivery of CRISPR-Cas9 ribonucleoprotein and selectable marker plasmid in rice.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 111 citations in OpenAlex.
- CRISPR RNP-Mediated Transgene-Free Genome Editing in Plants: Advances, Challenges and Future Directions for Tree Species.Plant, cell & environment · 2026Review
- Article
- Genome editing beyond transgenes: DNA-free technologies in crop improvement.Frontiers in plant science · 2026Review
- Efficient production of gene-edited onion (Allium cepa) plants using biolistic delivery of cas9 RNPs and transient expression constructs.Plant cell reports · 2025Article
- CRISPR/Cas system-mediated transgene-free or DNA-free genome editing in plants.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Review
- Enhancing biolistic plant transformation and genome editing with a flow guiding barrel.Nature communications · 2025Article
- An efficient mRNA delivery system for genome editing in plants.Plant biotechnology journal · 2025Article
- A long journey towards genome editing technologies in plants: a technical and critical review of genome editing technologies.Frontiers in genome editing · 2025Review
- CRISPR-based gene editing in plants: Focus on reagents and their delivery tools.BioImpacts : BI · 2025Review
- Reverse Mutations in Pigmentation Induced by Sodium Azide in the IR64 Rice Variety.Current issues in molecular biology · 2024Article
- Development of a CRISPR/Cas12a-based fluorescent detection method of Senecavirus A.BMC veterinary research · 2024Article
- Review
- Decoding the complexity of on-target integration: characterizing DNA insertions at the CRISPR-Cas9 targeted locus using nanopore sequencing.BMC genomics · 2024Article
- Editing of the ethylene biosynthesis gene in carnation using CRISPR-Cas9 ribonucleoprotein complex.Plant methods · 2024Article
- A set of vectors and strains for chromosomal integration in fission yeast.Scientific reports · 2023Article
- Review
- Microparticle-mediated CRISPR DNA delivery for genome editing in poplar.Frontiers in plant science · 2023Article
- A perspective from the EU: unintended genetic changes in plants caused by NGT-their relevance for a comprehensive molecular characterisation and risk assessment.Frontiers in bioengineering and biotechnology · 2023Review
- General guidelines for CRISPR/Cas-based genome editing in plants.Molecular biology reports · 2022Review
- CRISPR/Cas9 System: A Potential Tool for Genetic Improvement in Floricultural Crops.Molecular biotechnology · 2022Review
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An important advantage of delivering CRISPR reagents into cells as a ribonucleoprotein (RNP) complex is the ability to edit genes without reagents being integrated into the genome. Transient presence of RNP molecules in cells can reduce undesirable off-target effects. One method for RNP delivery into plant cells is the use of a biolistic gun. To facilitate selection of transformed cells during RNP delivery, a plasmid carrying a selectable marker gene can be co-delivered with the RNP to enrich for transformed/edited cells. In this work, we compare targeted mutagenesis in rice using three different delivery platforms: biolistic RNP/DNA co-delivery; biolistic DNA delivery; and Agrobacterium-mediated delivery. All three platforms were successful in generating desired mutations at the target sites. However, we observed a high frequency (over 14%) of random plasmid or chromosomal DNA fragment insertion at the target sites in transgenic events generated from both biolistic delivery platforms. In contrast, integration of random DNA fragments was not observed in transgenic events generated from the Agrobacterium-mediated method. These data reveal important insights that must be considered when selecting the method for genome-editing reagent delivery in plants, and emphasize the importance of employing appropriate molecular screening methods to detect unintended alterations following genome engineering.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.