ArticleScientific reports2019
A CRISPR-Cas9 System for Genome Editing of Fusarium proliferatum.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 67 citations in OpenAlex.
- A streamlined protocol for small-scale protoplast generation and CRISPR/Cpf1-mediated genome editing iniScience · 2026Article
- Climate-Driven Regulatory Plasticity Reshapes Mycotoxin Risk and Control in Food Systems.Comprehensive reviews in food science and food safety · 2026Review
- Advancements in CRISPR-Cas Systems for Genome Editing towards Eradication of Human Microbial Pathogens.Molecular biotechnology · 2026Review
- Genetic and molecular approaches for Fusarium wilt resistance in garden pea: advances and future outlook.Plant molecular biology · 2025Review
- CRISPR/Cas9-assisted gene editing reveals that EgPKS, a polyketide synthase, is required for the biosynthesis of preussomerins in Edenia gomezpompae SV2.World journal of microbiology & biotechnology · 2025Article
- Unlocking Fungal Potential: The CRISPR-Cas System as a Strategy for Secondary Metabolite Discovery.Journal of fungi (Basel, Switzerland) · 2024Review
- A combined transcriptomic and physiological approach to understanding the adaptive mechanisms to cope with oxidative stress inMicrobiology spectrum · 2023Article
- CRISPR/Cas9 mediated gene editing of transcription factor ACE1 for enhanced cellulase production in thermophilic fungus Rasamsonia emersonii.Fungal biology and biotechnology · 2023Article
- The Microbial Connection to Sustainable Agriculture.Plants (Basel, Switzerland) · 2023Review
- Virulence and antibiotic-resistance genes in Enterococcus faecalis associated with streptococcosis disease in fish.Scientific reports · 2023Article
- A Case of Laying Hens Mycosis Caused byVeterinary medicine international · 2023Article
- Evasion of Cas9 toxicity to develop an efficient genome editing system and its application to increase ethanol yield in Fusarium venenatum TB01.Applied microbiology and biotechnology · 2022Article
- Genome editing using a versatile vector-based CRISPR/Cas9 system in Fusarium species.Scientific reports · 2022Article
- CRISPR/Cas9 using a transient transformation system in Ceriporiopsis subvermispora.Applied microbiology and biotechnology · 2022Article
- CRISPR/Cas9 and Nanotechnology Pertinence in Agricultural Crop Refinement.Frontiers in plant science · 2022Review
- CRISPR/Cas9 RNP-assisted validation of palmarumycin biosynthetic gene cluster inFrontiers in microbiology · 2022Article
- Article
- Gene editing enables rapid engineering of complex antibiotic assembly lines.Nature communications · 2021Article
- CRISPR/Cas9 mediated editing of the Quorn fungus Fusarium venenatum A3/5 by transient expression of Cas9 and sgRNAs targeting endogenous marker gene PKS12.Fungal biology and biotechnology · 2021Article
- Current Approaches for Advancement in Understanding the Molecular Mechanisms of Mycotoxin Biosynthesis.International journal of molecular sciences · 2021Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fusarium proliferatum causes diverse diseases of many economically important plants. The fungus produces several mycotoxins of which the fumonisins are the most toxic. Currently, deletion of key genes for mycotoxin biosynthesis is a laborious and time-consuming procedure. We developed a novel CRISPR/Cas9-based genome-editing tool for the direct delivery of preassembled Cas9 ribonucleoproteins into protoplasts of F. proliferatum. Our CRISPR-Cas9 system couples a site-specific double-strand DNA break mediated by two Cas9 ribonucleoproteins with microhomology recombination requiring only 50-bp regions flanking the target gene. This system reduces the risk of off-target mutations and minimizes the risk of altering any gene adjacent to the target region. We used this tool to delete a polyketide synthase gene (FUM1) required for fumonisin biosynthesis. The mutants generated are no longer able to produce fumonisins, confirming the key role of FUM1 in fumonisin biosynthesis. Our CRISPR-Cas9 system is an important new tool for genetic studies of Fusarium.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.