Evidence map›Paper›PMID 31881209›Full record

ArticleJournal of molecular biology2020

The Staphylococcus aureus IsdH Receptor Forms a Dynamic Complex with Human Hemoglobin that Triggers Heme Release via Two Distinct Hot Spots.

Ken Ellis-Guardiola, Joseph Clayton, Clarissa Pham, Brendan J Mahoney, Jeff Wereszczynski, Robert T Clubb

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of molecular biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
1.3field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 22 citations in OpenAlex.

  1. Cryo-EM provides insight into how thebioRxiv : the preprint server for biology · 2026
    Article
  2. Article
  3. Article
  4. Hemoglobin receptor redundancy inJournal of structural biology: X · 2025
    Article
  5. Article
  6. Molecular basis of hemoglobin binding and heme removal inProceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  7. Review
  8. The Shr receptor fromProceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  9. Article
  10. Article
  11. Cryo-EM structures of staphylococcal IsdB bound to human hemoglobin reveal the process of heme extraction.Proceedings of the National Academy of Sciences of the United States of America · 2022
    Article
  12. Article
  13. Review
  14. Article
  15. Review
  16. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Ken Ellis-GuardiolaUCLA Department of Chemistry and Biochemistry, University of California, Los Angeles, 611 Charles Young Drive East, Los Angeles, CA 90095, USA; UCLA-DOE Institute of Genomics and Proteomics, University of California, Los Angeles, 611 Charles Young Drive East, Los Angeles, CA 90095, USA.
Joseph ClaytonDepartment of Physics and Center for Molecular Study of Condensed Soft Matter, Illinois Institute of Technology, Chicago, IL 60616, USA.
Clarissa PhamUCLA Department of Chemistry and Biochemistry, University of California, Los Angeles, 611 Charles Young Drive East, Los Angeles, CA 90095, USA; UCLA-DOE Institute of Genomics and Proteomics, University of California, Los Angeles, 611 Charles Young Drive East, Los Angeles, CA 90095, USA.
Brendan J MahoneyUCLA Department of Chemistry and Biochemistry, University of California, Los Angeles, 611 Charles Young Drive East, Los Angeles, CA 90095, USA; UCLA-DOE Institute of Genomics and Proteomics, University of California, Los Angeles, 611 Charles Young Drive East, Los Angeles, CA 90095, USA.
Jeff WereszczynskiDepartment of Physics and Center for Molecular Study of Condensed Soft Matter, Illinois Institute of Technology, Chicago, IL 60616, USA.
Robert T ClubbUCLA Department of Chemistry and Biochemistry, University of California, Los Angeles, 611 Charles Young Drive East, Los Angeles, CA 90095, USA; UCLA-DOE Institute of Genomics and Proteomics, University of California, Los Angeles, 611 Charles Young Drive East, Los Angeles, CA 90095, USA; Molecular Biology Institute, University of California, Los Angeles, 611 Charles Young Drive East, Los Angeles, CA 90095, USA. Electronic address: rclubb@mbi.ucla.edu.
University of California, Los Angeles · USIllinois Institute of Technology · US

Funding

Cell Surface Protein Anchoring in Gram-Positive BacteriaR01AI052217 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Robert Thompson Clubb · 2002 to 2026
$8.5M
Probing the Structure/Function/Dynamics Relationship in Biomolecular Complexes With Multiscale Computational TechniquesR35GM119647 · NIGMS · ILLINOIS INSTITUTE OF TECHNOLOGY · PI WERESZCZYNSKI, JEFFERY · 2016 to 2025
$3.8M
Molecular basis of heme scavenging by Gram-positive bacteriaR01AI161828 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Robert Thompson Clubb · 2021 to 2026
$3.0M
Development of a cell-based assay to discover new bacterial anti-infective agentsR01AI121360 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI CLUBB, ROBERT THOMPSON · 2016 to 2018
$1.1M
Stopped-Flow SpectrometerS10OD023584 · OD · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI YEATES, TODD O · 2017 to 2017
$106k
NIAID NIH HHS R01 AI052217NIAID NIH HHS R01 AI121360NIAID NIH HHS R01 AI161828NIGMS NIH HHS R35 GM119647NIH HHS S10 OD023584
6 · The paper itself

Abstract

Iron is an essential nutrient that is actively acquired by bacterial pathogens during infections. Clinically important Staphylococcus aureus obtains iron by extracting heme from hemoglobin (Hb) using the closely related IsdB and IsdH surface receptors. In IsdH, extraction is mediated by a conserved tridomain unit that contains its second (N2) and third (N3) NEAT domains joined by a helical linker, called IsdH

Indexed as

Antigens, BacterialBacterial ProteinsHemeHemoglobinsHumansIronMagnetic Resonance SpectroscopyMolecular Dynamics SimulationProtein BindingProtein Structure, SecondaryReceptors, Cell SurfaceSpectrophotometryStaphylococcus aureusAntigens, BacterialBacterial ProteinsHemeHemoglobinsIronIsdH protein, Staphylococcus aureusReceptors, Cell SurfaceIron-regulated surface determinant systemMolecular dynamicsNuclear magnetic resonance spectroscopyStaphylococcus aureus hemoglobin receptorStopped-flow spectrophotometry

Identifiers

PMID31881209
PMCPMC7309296
OpenAlexW2996966858

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.