ArticleDevelopmental biology2020
The cytoskeletal motor proteins Dynein and MyoV direct apical transport of Crumbs.
Article in Developmental biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 27 citations in OpenAlex.
- Adherent junctions: Physiology, role in hydrocephalus and potential therapeutic targets.IBRO neuroscience reports · 2025Review
- CRB1 is required for recycling by RAB11A+ vesicles in human retinal organoids.Stem cell reports · 2023Article
- AAV-mediated gene augmentation therapy of CRB1 patient-derived retinal organoids restores the histological and transcriptional retinal phenotype.Stem cell reports · 2023Article
- Phase separation of Hippo signalling complexes.The EMBO journal · 2023Article
- Rab11 negatively regulates wingless preventing JNK-mediated apoptosis in Drosophila epithelium during embryonic dorsal closure.Cell and tissue research · 2023Article
- Structure Composition and Intracellular Transport of Clathrin-Mediated Intestinal Transmembrane Tight Junction Protein.Inflammation · 2023Review
- RAB6 and dynein drive post-Golgi apical transport to prevent neuronal progenitor delamination.EMBO reports · 2022Article
- Actomyosin activity-dependent apical targeting of Rab11 vesicles reinforces apical constriction.The Journal of cell biology · 2022Article
- Tissue architecture: Two kinesins collaborate in building basement membrane.Current biology : CB · 2022Article
- RASSF8-mediated transport of Echinoid via the exocyst promotes Drosophila wing elongation and epithelial ordering.Development (Cambridge, England) · 2021Article
- Drosophila USP22/nonstop polarizes the actin cytoskeleton during collective border cell migration.The Journal of cell biology · 2021Article
- Regulation of apical constriction via microtubule- and Rab11-dependent apical transport during tissue invagination.Molecular biology of the cell · 2021Article
- Research Models and Gene Augmentation Therapy forFrontiers in neuroscience · 2020Review
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Crumbs (Crb in Drosophila; CRB1-3 in mammals) is a transmembrane determinant of epithelial cell polarity and a regulator of Hippo signalling. Crb is normally localized to apical cell-cell contacts, just above adherens junctions, but how apical trafficking of Crb is regulated in epithelial cells remains unclear. We use the Drosophila follicular epithelium to demonstrate that polarized trafficking of Crb is mediated by transport along microtubules by the motor protein Dynein and along actin filaments by the motor protein Myosin-V (MyoV). Blocking transport of Crb-containing vesicles by Dynein or MyoV leads to accumulation of Crb within Rab11 endosomes, rather than apical delivery. The final steps of Crb delivery and stabilisation at the plasma membrane requires the exocyst complex and three apical FERM domain proteins - Merlin, Moesin and Expanded - whose simultaneous loss disrupts apical localization of Crb. Accordingly, a knock-in deletion of the Crb FERM-binding motif (FBM) also impairs apical localization. Finally, overexpression of Crb challenges this system, creating a sensitized background to identify components involved in cytoskeletal polarization, apical membrane trafficking and stabilisation of Crb at the apical domain.
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