ArticleMicrobial biotechnology2020
An Ustilago maydis chassis for itaconic acid production without by-products.
Article in Microbial biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Precise Engineering of Sustainable Fiber Composites Comprising Biobased or Valorized Matrices and Solid Components: A Perspective.ACS environmental Au · 2026Review
- FLP/FRT-Mediated Recombination for Resistance Marker Recycling.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Isolation of Genomic DNA from Ustilago Species.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Low Oxygen Availability Increases Itaconate Production by Ustilago maydis.Biotechnology and bioengineering · 2025Article
- What are the 100 most cited fungal genera?Studies in mycology · 2024Article
- Application of valencene and prospects for its production in engineered microorganisms.Frontiers in microbiology · 2024Review
- Development of an itaconic acid production process with Ustilaginaceae on alternative feedstocks.BMC biotechnology · 2023Article
- Huitlacoche (Molecules (Basel, Switzerland) · 2023Review
- Itaconic acid production is regulated by LaeA inMetabolic engineering communications · 2022Article
- Article
- Versatile CRISPR/Cas9 Systems for Genome Editing inJournal of fungi (Basel, Switzerland) · 2021Article
- Ustilaginaceae Biocatalyst for Co-Metabolism of COJournal of fungi (Basel, Switzerland) · 2021Article
- An OptimizedJournal of fungi (Basel, Switzerland) · 2020Article
- Consolidated bioprocessing of cellulose to itaconic acid by a co-culture of Trichoderma reesei and Ustilago maydis.Biotechnology for biofuels · 2020Article
- An Ustilago maydis chassis for itaconic acid production without by-products.Microbial biotechnology · 2020Article
- GC-MS-Based Metabolomics for the Smut FungusFrontiers in molecular biosciences · 2020Article
- Article
- Development and Application of CRISPR/Cas in Microbial Biotechnology.Frontiers in bioengineering and biotechnology · 2020Review
- Establishing Polycistronic Expression in the Model MicroorganismFrontiers in microbiology · 2020Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ustilago maydis is a promising yeast for the production of a range of valuable metabolites, including itaconate, malate, glycolipids and triacylglycerols. However, wild-type strains generally produce a potpourri of all of these metabolites, which hinders efficient production of single target chemicals. In this study, the diverse by-product spectrum of U. maydis was reduced through strain engineering using CRISPR/Cas9 and FLP/FRT, greatly increasing the metabolic flux into the targeted itaconate biosynthesis pathway. With this strategy, a marker-free chassis strain could be engineered, which produces itaconate from glucose with significantly enhanced titre, rate and yield. The lack of by-product formation not only benefited itaconate production, it also increases the efficiency of downstream processing improving cell handling and product purity.
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Registered trials
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